STUDIES ON MOLECULAR MECHANISMS AND PHYSIOLOGICAL FUNCTIONS OF ANTI-CELL ADHESION

一些已建立的细胞系对硫酸软骨素蛋白多糖的抗粘附活性不敏感，而另一些细胞系则存在膜联蛋白VI的缺陷，膜联蛋白VI是硫酸软骨素链具有抗粘附活性的候选受体。这些可能有助于分析膜联蛋白VI在抗粘附中的可能作用（Kimata）。用反义RNA转染HSP-47表达，不仅导致胶原合成减少，而且导致胶原不溶化，这表明HSP-47起着shaperon的作用（Nagata）。Kitagawa分析了转染突变层粘连蛋白基因对细胞行为的影响。高表达tenascin的乳腺癌细胞与低表达tenascin的乳腺癌细胞相比，细胞形态呈圆形，细胞迁移活性高，侵袭性强，提示tenascin的抗粘附活性对转移有影响（Sakakura）。此外，与抗黏附不同的是，rgd不依赖于…更多的凹痕整合素可能参与腱蛋白的隐细胞黏附活性（Obara）。神经聚糖C是一种新型的膜结合蛋白多糖，可影响大鼠脑细胞粘附并可能诱导神经突生长（Oohira）。低转移活性的肺癌细胞通常表现为syndecan的高表达，并倾向于表现为有组织的应激纤维的形成，而高活性的肺癌细胞则倾向于诱导皱褶膜的形成。这种差异可能解释了细胞粘附性和癌细胞转移能力之间的可能关系（Okayama）。Sekiguchi发现纤维连接蛋白的选择性剪接调节了细胞粘附的能力。一种具有酪氨酸磷酸酶活性的脑蛋白多糖已被发现对神经中枢的神经元细胞具有抗黏附活性。这种蛋白多糖可能作为多营养蛋白的受体（Noda）。Sofue认为层粘连蛋白可能是一种依赖igf -i的分化诱导因子，并进一步发现酪氨酸磷酸化参与信号转导。Inagaki成功地提高了对磷酸化的细胞骨架蛋白的单克隆抗体，并揭示了中间丝磷酸化与增殖细胞粘附变化的可能关系。Tsukita观察到，当钙粘蛋白结合蛋白b-连环蛋白被迫表达时，整合素会被激活。第一次和第二次小组会议在名古屋举行，讨论成果，相互提出有益建议和意见，交流最新信息。少

Some of established cell lines are insensitive to anti-adhesive activity of chondroitin sulfate proteoglycans, and other lines are defective of annexin VI which is a candidate of the receptor for chondroitin sulfate chains with anti-adhesive activity. Those may be useful to analyze a possible role of annexin VI in anti-adhesion (Kimata). Transfection with antisense RNA for HSP-47 expression caused not only the decrease in collagen synthesis but also insolubilization of the collagen, which suggests a role of HSP-47 as shaperon (Nagata). Kitagawa analyzed the effect of the transfection with mutant laminin genes on the cell behaviors. Mammary carcinoma cells with high activity of tenascin production, compared to those with the low activity, showed the round cell morphology, the high activity of cell migration and the high invasiveness, which sugggests the effect of anti-adhesive activity of tenascin on the metastasis (Sakakura). In addition, differently from the anti-adhesion, RGD-indepen … More dent integrin may be involved in a cryptic cell-adhesive acitivity of tenascin (Obara). A novel membrane-bound proteoglycan, neuroglycan C influences cell adhesion in rat brain and may induce the neurite outgrowth (Oohira). lung carcinoma cells with the low metastatic activity usually show the high expression of syndecan and tend to show the organized stress fiber formation, but those with the high activity tend to induce the formation of ruffling membranes. The difference may explain a possible realationship between the cell adhesiveness and the metastatic ability of the carcinoma (Okayama). Sekiguchi has found that altemative splicing of fibronectin modulates the capacity for cell-adhesion. A brain proteoglycan with tyrosine phosphatase activity has been found to exhibit anti-adhesion activity to neuronal cells in the nervous center. This proteoglycan may function as a receptor for pleiotrophin (Noda). Sofue has suggested that laminin may be a IGF-I-dependent differentiation-inducing factor and further found the inbolvement of tyrosine phosphorylation in the signal transduction. Inagaki has succeeded in raising monoclonal antivodies to phosphorylated cytoskeletal proteins and revealed the possible relationship of the phosphorylation of intermediate filaments to the changes in cell-adhesion of proliferating cells. Tsukita has observed the activation of integrin when a cadherin-binding protein, b-catenin is forced to be expressed. The first and second group meetings were held in Nagoya to discuss results, give useful suggestions and comments each other and exchange current informations. Less