Medical application of Polymer Electrolyte Complek to reconstruction of the alveoler bone and dental implant.

本研究的目的是利用聚合物电解质复合物这种新材料，在老化和某些疾病的过程中，将新的骨组织重建到牙吸收床周围的支撑牙槽骨中，缩短骨组织与种植体融合的时间。我们首先用6种聚阳离子和15种聚阴离子制备了90种不同的PEC膜。根据成骨细胞、牙周细胞和上皮细胞的细胞间黏附试验和增殖试验以及成骨细胞的碱性磷酸酶和胶原合成活性，从所有PEC膜中选择8种有潜力的材料进行进一步的实验。将与PEC或HA杂交的两种膜植入骨内的体内植入实验结果发现，8种PEC膜中的两种材料均具有良好的体内亲和性，具有良好的骨关节炎。体内种植实验也显示，涂覆PEC材料的两种牙种植体诱导骨形成的速度明显快于对照种植体。当这些牙种植体作为BMP的载体物质时，也会诱发异位成骨。这表明它们可以用作载体。这些结果表明，这两种PEC物质可用于骨缺损的填充和牙种植体的涂层。因此，这些物质是有望投入医疗实践的新材料。

The pur pose of this study is to, using a new material, Polymer Electrolyte Complex reconstruct a new bone tissue into the suppor ting alveolar bone around teeth absor bed in a process of aging and some diseases and shorten a period which requires the fusion of a bone tissue with a dental implant.We first prepared 90 different PEC membranes, using 6 kinds of polycations and 15 polyanions. Eight promising materials of all the PEC membranes were selected for further experiments from the results of cell-to-cell adhesion tests and proliferation assays of osteogenic, periodontal and epithelial cells as weii as ALP and collagen synthetic activities of osteogenic cells.Two materials of the eight PEC membranes, all of which showed a good in vivo affinity, were found to have an excellent osteoacutis from the results of the in vivo implantation experiment in which the two membranes hybridized with PEC or HA were embeddedinto bones.The in vivo implantation experiment also revealed that the two dental implants coated with the PEC materials could induce significantly more rapid bone formation than the control implants. These dental implants, when used as a carrier substance for BMP,also induced the ectopic osteogenesis. This indicates that they could be used as a carrier. These observations show that the two PEC substances could be useful for bone defect filling and coating dental implants. Thus, these substances are promising new materials that would be put into medical practice.