Analytical system of free moving-exercise for animal on an experimental1 basis.

最近，缺乏锻炼和饮食习惯西化对以健康状况为基础的日常生活城市化的影响在日本受到关注。另一方面，营养摄入不均衡的体育俱乐部学生的健康状况也得到了改善。设计了一套大鼠自由活动系统，即4只大鼠(80m*20 cm*25 cm)通过3根管子(内径10 cm)纵向排列，并在楼上设置饮用水，在地下设置饮食。在自由流动体系(Ex+)和常规体系(Ex-)中，分别饲喂低镁(Mg1/3)饲料和正常饲料(mg+)，共4组(1)Ex-Mg+，2)Ex+Mg+，3)Ex-Mg-，4)Ex+Mg-。实验4组大鼠肌肉重量比实验1组大鼠减轻。取材后，测定血浆生化指标和肝脏组织中总脂质、过氧化脂质、蛋白质和超氧化物歧化酶(总-SOD、Mn-SOD)的含量。第4组血浆ALB、AMY、LDH、CPK、UA、CRE显著低于第1组，PL、T-CHO、ALB、ALP、UA显著低于第2组，第3组UA显著低于第1组，PL、ALB、ALP、CHE、UA显著低于第2组。这些结果表明，低镁营养状态下的健康状况会因运动而下降。

Recently, effects of lack of exercise and westernizing of food habits on health status based urbanizing of daily life are focused in Japan. On the other hand, health status of students belonged to an athletic club under unbalanced nutritional in take are also picked up. A free moving system was designed for exercise of rats, that is 4 case (80 m * 20 cm * 25 cm) were arranged lengthways through three pipes (10 cm ID), and drinking water on upstairs and diets were set up under ground. In the free moving system (Ex+) or the conventional system (Ex-), rats fed low magnesium (Mg1/3) diet or normal diet (Mg+), (total 4 groups ; 1)Ex-Mg+, 2)Ex+Mg+, 3)Ex-Mg-, 4)Ex+Mg-) were kept for 40 days. Muscle weight decreased in rats of group 4 compared to group 1.After sampling, biochemical items in plasma, and total lipid, lipid peroxide, protein and superoxide dismutase (total-SOD,Mn-SOD) in liver were measured. In plasma of group 4, ALB,AMY,LDH,CPK,UA & CRE were significantly low compared to that of group 1 and PL,T-CHO,ALB,ALP & UA in group 4 were low compared to that of group2.In plasma of group 3, UA was significantly low compared to that of group 1 and PL,ALB,ALP,CHE & UA were low compared to group 2.In lipid peroxide of liver of groups 1 & 2 was significantly high compared to that of other groups. These results suggested that health status under low Mg nutrition would be fallen down by exercise.