Development of melanogenesis inhibitors available for skin bleaching.

本研究旨在寻找天然和农产品中的化合物，用于漂白或治疗黄褐斑和老年性色素沉着患者的皮肤色素沉着。在我们的筛选系统中，发现大米提取物对黑素生成的抑制作用，以筛选非致黑素化合物。将大米全粒粉碎，用水浸泡，在50ﾟC下浸提1天以上，浸提液加热过滤。提取物用蒸发器浓缩，并应用于各种柱层析，如Sephadex G-25，MCL Gel和Kiseel Gel。得到的组分在高倍液相色谱中最终呈现单峰，我们将其命名为分离物A，它降低了培养的B-16小鼠黑色素瘤细胞的酪氨酸酶活性和黑素合成。但分离株A不影响蛋白质合成等细胞活性，选择性地阻断酪氨酸酶蛋白的合成，目前尚不清楚分离株A的提取物化学成分及其与黑素生成抑制过程的关系，因此还需要更多的研究。我们希望A分离物能够应用于美容或医疗。

Our research is to find the compound from natural and farm products for external application to bleach or cure skin pigmentation of the patient with melasma and senile pigmentation.The inhibitive ability of rice extract on melanogenesis was discovered in our screening system to select untimelanogenic compounds. Whole grains of rice, Oryza sative were pulverized, soaked in water, and extracted over one day at 50ﾟC.The extract solution was heated and filtered. the extract was enriched using an evaporator, and it was applied on various colum ahromatographies such as Sephadex G-25, MCL Gel and Kiesel Gel. Obtained fraction finally show a single peak in the high power liquid chromatography, and we named it "Isolation A"Isolation A decreased tyrosinase activity and melanogenesis in B-16 mouse melanoma cells in culture. However, it did not affect the activity of cells such as protein synthesis, which iondicates Isolation A selectively blocked the synthesis of tyrosinase protein.The extract chemical composition of Isolation A and the relationship between Isolation A and the inhibition process of melanogenesis are not known at present, and therefore more studies are required. We hope that Isolation A will be able to be applied for cosmetic or medical treatment.