Fate of a specific microorganism in soil.

(1)利用全细胞杂交技术检测番茄青枯病致病菌番茄伯克氏菌（Burkholderia solanacearum）细胞：利用DNA数据库研究番茄伯克氏菌核糖体特异性DNA序列。利用检测探针对纯培养的茄芽孢杆菌细胞进行特异性染色。此外，在番茄根茎中也能检测到这些细胞。(2)番茄芽孢杆菌在番茄根际的行为。1)研究番茄枯萎菌接种土壤时土壤水分和温度条件对番茄枯萎病的防治效果。2)采用平板计数法，研究了番茄土壤中茄芽孢杆菌的种群动态。在抑制土壤中，根际、根面和根内的生长受到的抑制比诱导土壤中更大。两种土壤的非根际土壤无明显差异。

(1) Detection of the cells of tomato bacterial wilt agent, Burkholderia solanacearum, by whole-cell hybridization : Ribosomal DNA sequence specific for B.solanacearum was investigated using DNA database. By using the detection probe, cells of B.solanacearum in pure culture can be stained specifically. In addition, the cells can be detected in tomato rhizoplane.(2) Behavior of B.solanacearum in tomato rhizosphere.1) Soil moisture condition and soil temperature condition were investigated for controlling tomato bacterial wilt when B.solanacearum was inoculated to soil.2) Population dynamics of B.solanacearum in tomato soil were investigated under the conditions obtained in 1) by plate counting. In the suppressive soil used, growth of B.solanacearum was suppressed in rhizosphere and rhizoplane and within the roots more than in the induced soil. No clear difference was observed in non-rhizosphere soil between the two soils.