Estimation of genetic diversity on the interspecific hybrid between Picea jezoensis and P.glehni.

本文主要从天然林杂交动态和人工杂交后代遗传两方面研究了云杉与格列匹斯杂交后代的表型遗传。为了阐明天然林杂交形成的过程，于1995年7月在东京大学北海道大学森林的天然林中设置了一个研究地块。小区面积为40m*40m。1995年6月发现2个杂交个体。物种的名字，DBH。测量了突现树种的高度，并绘制了各树种的位置图。杂交种分布在纯云杉林和以沙卡林冷杉和干果冷杉为主的混交林之间的湿林分上。比较两种云杉及其杂交种的形态，格列云杉的形态为方形，干热云杉的形态为扁平，杂交种的形态为白蚁状。明确表型遗传模式。对叶片外形进行了试验研究。芽开放的时间，以及对蚜虫的耐受性。由于空山云杉和格林尼云杉的发芽时间不同，对开花时间的影响也不同，因此对阐明杂交形成机制具有重要意义。在草试上考察了芽开放时间。空肠云杉（Picea jezoensis）的变异是一次性完成的，而格伦云杉（Picea glehni）的变异是种内变异。该杂种在芽张开上表现出间歇性。在蚜虫危害方面，干云杉（Picea jezoensis）被蚜虫侵害较多，而云杉（Picea glehni）被蚜虫侵害较少。杂交种在树数和受害程度上均有不同程度的损害。高个子的人受到的伤害尤其严重。此外，还从天然林研究地块的树木叶片中提取了DNA。用RAPD标记对个体进行分析。一些随机引物被认为是物种特异性的，可以用来检验假设的杂交。少

The phenotype inheritance on the hybrid between Picea jezoensis and Pices glehni was studied especially reference to 1) dynamics of hybrid in the natural forest and 2) inheritance among artificial cross progeny. In order to clorify the process of hybrid formation in the natural forest, a study plot was set in the natural forest in the University Forest in Hokkaido, University of Tokyo in July, 1995. The plot size was 40m*40m. The plot included two hybrid individuals which were found in June, 1995. The name of species, DBH.height of emergent tree species were measured and the location of each tree was mapped also. The hybrids located on wet stand between the pure Picea glehni forest and the mixed stand dominated with Abies sachalinensis and Pices jezoensis. Compared among the profile of two Pices species and their hybrid, that shape of Picea glehni was square, that of Pices jezoensis was flat, and that of hybrid was in termittent.To make clear the inheritance pattern of phenotype. the c … More ross trial was investignted on the shape of leaf profile. time of bud opening, and the tolerance to the aphid. The time of bud opening is very important to clarify the mechanism of hybrid formation because that time is different between Picea jezonsis and Picea glehni and effects on the bloom time. The time of bud opening was investigated on the crass trial. That of Picea jezoensis was finished at once although intra-species variation occurred in that of Picea glehni. The hybrid showed intermittent trait on bud opening. As to the damage caused by aphid, lots of trees of Picea jezoensis were attacked by aphid, but little were attacked of Picea glehni. The hybrids were damuged in the number of trees as well as the degree of damage. Tall indivlduals were especially damaged.Furthermore, DNA was extracted from leaves of trees in the study plot in the natural forest. The individuals were tried to be analyzed with RAPD marker. Some of the random primer were thought to be species specific and be useful to check the putative hybrid. Less