DETERMINATION OF STRUCTURE OF AMYLOIDOGENIC IMMUNOGLOBULIN LIGHT CHAIN AND APPLICATION TO MOLECULAR DIAGNOSIS

淀粉样蛋白纤维在水中提取，并用7M尿素溶解。主肽经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法分离后转移到PVDF膜上。从膜上切下主要多肽，进行N-端氨基酸序列分析。1例全身性累及的肺结节淀粉样变性为AMBDA III型AL-淀粉样变，1例局限性扁桃体淀粉样变性为LAMBDA VI型AL-淀粉样变。1例家族性淀粉样变伴多发性神经病，其30位由转甲状腺素变异体取代亮氨酸组成的淀粉样纤维。肝脏在炎症部位的细胞因子诱导产生SAA等急性时相蛋白，并进入血液循环。它们到达炎症部位，对抗组织损伤，抑制炎症。为了探讨HPV与子宫颈和头颈部肿瘤发生的关系，我们从肿瘤组织中提取DNA，用PCR方法扩增HPV基因组，并用原位杂交(ISH)检测HPV基因组在肿瘤中的定位。在宫颈恶性病变中，约90%检出恶化型HPV基因组，其中大部分未检出。在头颈部，食道鳞状细胞癌和扁桃体鳞状细胞癌分别含有HPV52b和HPV16。我们报告了中耳乳头状腺癌、腮腺转移癌、13年来多次复发的小涎腺透明细胞癌、声门下鳞状细胞癌伴甲状腺血行转移和颞骨疣状癌的病例。

Amyloid fibrils were extracted in water and dissolved in 7M urea. Main paptide was separated with SDS polyacrylamide gel electrophoresis and transfered on PVDF membrane. Main peptides were cut from the membrane and subjected to N-terminal amino acid sequence analysis. A pulmonary nodular amyloidosis with systemic involvement was determined as lambda III type AL-amyloidosis, while a tonsillar amyloidosis of localized type was lambda VI type AL-amyloidosis.A case of familial amyloidosis with polyneuropathy had amyloid fibrils composed of transthyretin variant with substitution leucine for valine at position 30.Acute phase proteins such as SAA are produced in liver induced by cytokines from inflammatory sites and enter into the blood circulation. They reach the inflammatory sites, act against tissue damage, and suppress the inflammation. I reviewed this system and summarized in a chapter of a book.To investigate a association of HPV with tumorgenesis in unterine cervix and head and neck region, we extracted DNA from tumors, amplyfied HPV genome with PCR method, and detected the localization of HPV genome in the tumor by in situ hybridazation (ISH). In malignant lesions of uterine cervix, HPV genomes of malignant type were detected in about 90% most of which were not detected in ISH.In head and neck, esophageal squamous cell carcinoma (SCC) and tonsillar SCC contained HPV52b and HPV 16 respectively.We reported cases of papillary adenocarcinoma of mid-ear, metastatic sebacious carcinoma to the parotid gland, hyalinizing clear cell carcinoma of minor salivcary gland which recurred several times in 13 years, subglottal squamous cell carcinoma with hematogenous metastatis to thyroid, and verrucous carcinoma of temporal bone.