Study of airway reactivity in chimera mouse

除了自主神经控制之外，气道反应性被认为是由各种细胞决定的，例如气道平滑肌细胞、上皮细胞、肺巨噬细胞、肥大细胞、嗜酸性粒细胞和淋巴细胞，它们是气道的组分。来自具有较高气道反应性的A/J小鼠和具有较低气道反应性的C3 H/He小鼠的F1小鼠显示较低的气道反应性，表明常染色体隐性遗传。因此，研究A/J和C3 H/He小鼠（A/J** C3 H）的嵌合体小鼠的气道反应性是非常有趣的。方法1）小鼠气道反应性的测定采用Konzett-Rossler法。2）嵌合小鼠：A/J和C3 H/He雌性小鼠腹腔注射人促性腺激素，与成年雄性小鼠同笼饲养。2天后，取出输卵管和子宫，用培养基冲洗输卵管，收集虫卵，培养。在8细胞期，将A/J和C_3H胚胎混合，加入植物血凝素（PHA）进行融合。将融合胚胎移植到人工受孕的ICR养母子宫内.结果3次实验均获得1只嵌合体小鼠（A/J** C3 H）。其气道对乙酰甲胆碱的反应性介于A/J和C_3H之间（分别为11.4U、10.9U和16.6U）。

In addition to autonomic nerve control, airway reactivity is thought to be determined by various cells such as airway smooth muscle cells, epithelial cells pulmonary macrophages, mast cells, eosinophils and lymphocytes which are components of the airway. Flmice from A/J mice with higher airway responsiveness and C3H/He mice with lower responsiveness were reported to show lower airway responsiveness, indicating the autosomal recessive heredity. Therefore, it is very interesting to examine the airway reactivity of the chimera mouse of A/J and C3H/He mouse (A/J**C3H).1. Methods1) Estimation of mouse airway reactivity was carried out according to Konzett Rossler method.2) Chimera mouse : A/J and C3H/He female mice were injected ip with human gonadtropin and caged with adult males. 2 days after, ovi duct and uteri were removed and the emfroys were collected by washing ovi duct by medium and cultured. At the 8-cell stage, A/J and C3H embroys were mixed and fused by adding phytohemagglutinin (PHA). The fused embroys were transferred to the uteri of pseudpregnant ICR foster mothers.2. ResultsFrom three experiments, only one chimera mouse (A/J**C3H) was produced. Its airway reactivity to methacholine was intermediate between values of A/J and C3H (11.4U,10.9U and 16.6U,respectively).The present study showed the chimera mouse is an experimental model of the lung transplantation.