Gut, Immune organ-mucosal immunity of gastro-intestinal tract-

背景和目的：为了阐明胃肠道在介导免疫中的作用，我们在1995年至1996年期间，使用细菌和病毒感染胃肠道上皮细胞的实验，研究了以下三点。(1)趋化因子家族的mRNA是否在上皮细胞中表达，（2）趋化因子mRNA是否翻译成蛋白质，以及（3）趋化因子蛋白质是否具有任何功能。因此，由于细菌或病毒感染，胃肠上皮细胞产生的趋化因子的模式彼此不同，这被认为是炎症细胞局部动员和炎症反应对各种感染的多样性的主要原因。此外，将从细胞培养物的上清液中获得的IL-8浓缩，然后添加到嗜中性粒细胞中并短暂培养。随后，通过流式细胞仪检测中性粒细胞表面CD 11b/18和Leu 8（L-选择素）的表达水平，结果显示， ...更多信息 提示CD 11b/18表达增加，L-选择素表达减少。而抗IL-8抗体部分抑制了这些升高和降低，提示细胞培养上清中的IL-8具有激活中性粒细胞的作用。1997年，我们用Ussing小室电生理分析了各种趋化因子和细胞培养上清对T84和/或18 CO（人胃肠道成纤维细胞）和/或中性粒细胞共培养系统的影响。此外，建立一个肥大细胞系的尝试，检查肥大细胞的影响，而不是neutrophils.Methods和结果：共培养的胃肠道上皮细胞与成纤维细胞促进膜去极化，与中性粒细胞共培养进一步促进去极化。用脐血单个核细胞加入IL-6、干细胞因子和前列腺素E_2建立肥大细胞系。我们打算进一步研究与此细胞株的共培养系统。少

Background and Objective : To clarify a role of the gastrointestinal tract in mediating immunity, we examined following three points between 1995 and 1996, using experiments where gastrointestinal epithelial cells are infected by bacteria and viruses. (1) Whether mRNA of the chemokine family is expressed in epithelial cells, (2) whether the chemokine mRNAs are translated into proteins and (3) whether the chemokine proteins have any functions. As a result, the patterns of chemokines produced by gastrointestinal epithelial cells due to bacterial or viral infections differed from each other, which was thought to be the main cause of the diversity in local mobilization of inflammatory cells and inflammatory responses against various infections. Furthermore, IL-8 obtained from supernatant of cell culture was concentrated, then added to neutrophils and briefly cultured. Subsequently, expression levels of CD11b/18 and Leu8 (L-selectin) on the surface of neutrophils were measured by FACS,resul … More ting in an increase in CD11b/18 and a decrease in L-selection expression. And anti-IL-8 antibody partially inhibited these increases and decreases, suggesting that IL-8 in supernatant of cell culture plays a role in activating neutrophils. However, other cytokines are also involved.Then in 1997, we used an Ussing chamber to electrophysiologically analyze the influence of various chemokines and supernatant of cell culture on T84 and/or 18CO (human gastrointestinal fibroblasts) and/or neutrophils coculture systems. In addition, establishment of a mast cell line was attempted, to examine the effects of mast cells instead of neutrophils.Methods and Results : Coculture of gastrointestinal epithelial cells with fibroblasts facilitated membrane depolarization, and coculture with neutrophils further facilitated depolarization. A mast cell line was established using a culture system where IL-6, stem cell factor and prostaglandin E_2 were added to mononuclear cells from cord blood. We intend to further investigate the coculture system with this cell lone. Less