hepatic is chemia on and reper Protectiue effect anti-adhesion molecules antibody ies on hepatic is chemia and reperfusion iniury, and its clinical application.

我们研究了粘附分子的单克隆抗体（mAb）对肝脏缺血-再灌注损伤、肝脏保存和内毒素休克诱导的肝损伤的疗效和枯否细胞功能的调节。虽然肝脏缺血90分钟不允许动物存活，但用ICAM-1和LFA-1的mAb预处理使存活率增加至57%。肝脏中性粒细胞的数量持续增加，直到90分钟缺血后再灌注24小时。mAb预处理可抑制再灌注后中性粒细胞的浸润和脂质过氧化物的升高，促进再灌注后肝ATP的恢复。在内毒素休克性肝损伤模型实验中也观察到类似的保护作用。在大鼠肝移植实验中，含有甲磺酸萘莫司他的新冲洗液和枯否细胞阻断剂发挥协同作用，导致冷保存肝移植后存活率增加。提示枯否细胞和中性粒细胞参与了肝脏缺血再灌注损伤和内毒素休克性肝损伤，应用抗黏附分子mAb预处理可有效预防缺血再灌注损伤和内毒素休克性肝损伤。

We investigated the efficacy of the monoclonal antibodies (mAb) to adhesion molecules and the modulation of Kupffer cell function on hepatic ischemia-reperfusion injury, liver preservation and endotoxin shock induced hepatic injury. Although ischemia of the liver for 90 min did not permit survival of the animals, pretreatment with mAb to ICAM-1 and LFA-1 increased the survival rate to 57%. The number of neutrophils in the liver increased continuously up to 24 hours after reperfusion following 90 min ischemia. Pretreatment with mAb suppressed the infiltration of neutrophils and the elevation of lipid peroxide, and enhanced the recovery of hepatic ATP after reperfusion. A similar protective results we also observed in the model experiment of endotoxin shock induced liver injury. In the experiment of rat liver transplantation, a new rinse solution containing nafamostat mesilate and Kupffer cell blockade exert synergistic effects, leading to increased survival after cold-preserved liver transplantation. These results suggest that Kupffer cell and neutrophils contribute to ischemia-reperfusion injury in the liver and endotoxin shocked induced liver injury, and the pretreatment with mAb to adhesion molecules are useful for prevention of ischemic liver cell injury and endotoxin shocked liver cell injury.