Analysis of Anti-Carbonic Anhydrase Antibody

本研究首先采用免疫印迹法和ELISA法检测了风湿性自身免疫病（RAD）患者血清中抗碳酸酐酶（CA）抗体的发生率。RAD患者血清中抗CA抗体检出率为20-30%，抗CA抗体与人CAI和CAII均呈反应性，并与临床症状进行了相关性研究。其次，利用合成的肽分析了被RAD血清或在健康个体中发现的天然自身抗体识别的CAI和CAII抗原上的表位。RAD患者的抗CA抗体识别CA氨基末端的α-螺旋部分和CA酶的活性结构域。这可能证明了抗CA抗体对CA活性的抑制作用，如前所述。与此相反，天然自身抗体只与CA的羧基端部分反应。这些观察可能提示RAD中的独特的自身免疫机制。我们还报告了一个抗CA抗体的患者，他有类似的CA缺乏症的症状。

At first, using immunoblot and ELISA,the incidence of anti-carbonic anlydrase (CA) antibodies in Rheumatic autoimmune disease (RAD) was investigated. Anti-CA antibodies were found in 20-30% of sera from patients with RAD.All anti-CA sera were reactive with both human CAI and CAII.The correlation of it with clinical syndrome has been investigated. Second, epitopes on CAI and CAII antigen which recognized by RAD sera or natural autoantibody found in healthy individuals were analyzed using synthesized peptides. Anti-CA antibodies in RAD patients recognized alfa-helical portion on amino-terminus of CA and active domains of CA enzyme. This may evident the inhibitory effect of anti-CA antibodies against CA activity which previously discussed. In contrast, natural autoantibodies exclusively reacted with a portion located at carboxy-terminus of CA.These observation may suggest the distinguished mechanism of autoimmunity in RAD.We also reported a patient with anti-CA antibody who has similar symptoms to CA deficiency.