Cell Biological Study for the Mechanism of Osteogenesis by Electromagnetic Fields.

实验结果如下：1.电磁场刺激对成骨细胞内钙离子掺入的影响电磁场刺激后的成骨细胞内钙离子浓度平均比对照组高15%。这种钙离子掺入的加速作用在频率为100赫兹、脉冲宽度为15秒、磁场强度为2.0高斯的电磁场中最为有效。2.细胞水平电磁场刺激促进成骨作用的体内评价。骨密度计测量的成骨能力在频率为100赫兹、脉冲宽度为15秒、磁场强度为2.0高斯的电磁场刺激下显著增加。这些结果反映了本研究在体外实验中获得的结果。在最佳电磁场刺激条件下，成骨细胞标志物碱性磷酸酶的活性和成骨细胞增殖的AgNOR染色结果显示，电磁场刺激促进成骨作用的机制是电磁场刺激改变了细胞对钙离子的摄取，通过细胞内信号传递诱导细胞合成碱性磷酸酶、细胞增殖等功能的改变，从而促进成骨作用。电磁场刺激条件改变了成骨细胞的成骨、生长和钙离子掺入，有力地提示了电磁场对细胞的影响。

The following cxperimental results were obtained by this research.1.Changes in Ca-ion incorporation into cells by stimulation of electromagnetic field in vitroIntracellular Ca-ion concentration in osteoblasts that underwent electromagnetic field stimulation was higher the that in control osteoblasts by 15% on average. This acceleration of Ca-ion incorporation was most efficient in the electromagnetic field of 100 Hz in frequency, 15 (sec in pulse width and 2.0 gauss of the magnetic field intensity.2.Evaluation of in vivo osteogenesis-enhancing effect of electromagnetic field stimulation at cellular levelOsteogenesis measured by an osteometer significantly increased under stimulation by the electromagnetic field of 100 Hz in frequency, 15 (sec in pulse width and 2.0 gauss of the magnetic field intensity. These results reflected those obtained in the in vitro experiment in the present study. In the examination of activity of alkaline phosphatase, a marker of osteoblast, and proliferation of osteoblasts using AgNOR staining, elevation of the enzyme and enhancement of proliferation were shown under the optimum stimulation conditions of the electromagnetic field.As the mechanism of enhancement of osteogenesis by stimulation with the electromagnetic field, these results demonstrated that Ca-ion uptake by cells was changed by the electromagnetic field stimulation, changes in functions at the cellular level such as synthesis of alkaline phosphatase and cell-proliferation were induced via intracellular signal transaction in osteoblasts, and osteogenesis was enhanced. The fact that osteogenesis, and growth and Ca-ion incorporation by osteoblasts were changed by conditions of electromagnetic field stimulation strongly suggests the effect of electromagnetic field on cells.