PRESENCE AND POSSIBLE FUNCTION OF GROWTH HORMONE BINDING PROTEIN IN HUMAN FOLLICULAR FLUID

为了阐明生长激素（GH）在卵巢卵泡发生中的作用，我们研究了体外受精患者血清和卵泡液中生长激素结合蛋白（GHBP）的存在及其可能的功能。主要结果如下：1. 取卵时血清和卵泡液中均存在3 ~ 32ng/ml的GHBP水平。2. 在每个患者的卵泡中GHBP的水平没有差异。血清GHBP水平与卵泡水平相当。3. 在卵巢过度刺激的过程中，血清中GHBP的水平相对稳定，尽管血清雌二醇水平上升。4. 血清GHBP水平（bbb20 ng/ml）较高的患者获得的卵母细胞数量显著低于血清GHBP水平（<20ng/ml）较低的患者（p<0.05）。5.我们用RT-PCR检测了卵母细胞采集时获得的颗粒细胞中生长激素受体mRNA的表达。然而，在研究的患者中，血清GHBP水平与颗粒细胞中生长激素受体的表达没有相关性。综上所述，GHBP可能通过调节血清和卵泡液中GH的作用而参与卵巢卵泡形成。血清GHBP水平可作为卵巢过度刺激患者卵巢储备的可能标志。

In order to elucidate the function of growth hormone (GH) in ovarian folliculogenesis, we studied presence and possible function of growth hormone binding protein (GHBP) in sera and follicular fluids of patients undergoing in vitro fertilization. The main results are as follows. 1. GHBP in the levels of 3-32ng/ml existed in the sera and follicular fluids at the time of oocyte pickup. 2. There was no difference in the levels of GHBP among follicles in each patient. The serum levels of GHBP equaled to those of follicles. 3. The levels of GHBP in the sera were relatively stable over the course of ovarian hyperstimulation despite the rising levels of serum estradiol. 4. The numbers of oocytes obtained in the patients with higher serum levels of GHBP (>20ng/ml) were significantly (p<0.05) lower than those of the patients with lower serum levels of GHBP (<20ng/ml). 5.By RT-PCR we detected growth hormone receptor mRNA in the granulosa cells obtained at the time of oocyte pickup. However, there was no correlation between the serum GHBP levels and the growth hormone receptor expression in the granulosa cells in the patients studied. In conclusion, GHBP may have a role in ovarian folliculogenesis by modulating GH action in sera and follicular fluid. The serum GHBP levels may serve as a possible marker of ovarian reserve in the patients undergoing ovarian hyperstimulation.