Experimental Study of Tooth Replantation

本研究的目的是评估四种溶液作为牙再植的储存介质对细胞内LDH活性、生长活性、人牙周韧带细胞（PDL细胞）形态变化和溶液渗透压的影响。四种测试溶液分别是ViaSpan、生理盐水、人静息唾液和巴氏奶。DMEM（含10% FBS）作为对照培养基。4种溶液和对照介质分别与PDL细胞在25℃下反应10、30、60和120分钟。溶液反应后立即测定细胞内LDH活性。通过恢复培养第1、2、4、7天的细胞计数来评价细胞的生长活性。在恢复培养的第1天和第7天用相差显微镜观察形态变化。在进行每次实验之前，用自动渗透计测量四种溶液和对照介质的渗透压。实验结果如下：1.实验结果表明：细胞内LDH活性：在所有放置时间，与ViaSpan和巴氏奶反应的组的活性高于与生理盐水和人静息唾液反应的组。与人类静息唾液发生反应的基团。在所有溶液中活性最低。生长活性：与ViaSpan、巴氏奶和生理盐水反应的组具有较高的活性。在与人静息唾液反应的组中，活性随反应时间的增加而降低。形态学变化：与ViaSpan和巴氏奶反应的组在所有反应时间几乎没有变化。随着反应时间的延长，与生理盐水反应的基团呈圆形。与人类静息唾液反应的基团随着反应时间的增加而出现萎缩和脱落。溶液渗透压：人静息唾液的渗透压是所有溶液中最低的。各溶液渗透压与细胞内LDH活性及牙周膜细胞生长活性均有相关性

The purpose of this study was to evaluate the effect of four solutions as storage media for tooth replantation on intracelluar LDH activity, growth activity, morphological changes of human periodontal ligament cells (PDL cells), and osmolality of solutions.The four test solutions were ViaSpan, physiological saline, human resting saliva and pasteurized milk. DMEM,including 10% FBS,was used as a control medium.The four solutions and control medium were allowed to react with PDL cells for 10,30,60 and 120 minutes respectively at 25ﾟC.The intracellular LDH activity was immediately measured after reaction of solutions. The growth activity was evaluated by the cell counts on days 1,2,4 and 7 of the recovery culture. The morphological changes were observed a phase-contrast microscope on days 1 and 7 of the recovery culture.Before performing each experiment, the osmolality of the four solutions and control medium were measured by an automatic osmometer.The results were as follows :1.The intrac … More ellular LDH activity : At all placement times, the activity of the group that reacted with ViaSpan and pasteurized milk were higher than the groups that reacted with physiological saline and human resting saliva. The groups that reacted with human resting saliva. had the lowest activity of all solutions.2.The growth activity : The groups that reacted with ViaSpan, pasteurized milk and physiological saline had high activity. In the groups that reacted with human resting saliva had the activity decreased with increasing reaction time.3.The morphological changes : The groups that reacted with ViaSpan and pasteurized milk showed almost no change in all reaction times. The groups that reacted with physiological saline became a rounded in appearance with increasing reaction time. The groups that reacted with human resting saliva appeared to shrivel and exfoliate with increasing reaction time.4.The osmolality of solution : The osmolality of human resting saliva was lowest of all solutions.5.There was a correlation among the osmolality of all solutions, the intracellular LDH activity and the growth activity of periodontal ligament cells Less