Studies on Bioactive Phenolic Compounds from Traditional Herbal Medicines.

1.抗肿瘤活性筛选试验（1）从络石中提取的木脂素牛蒡苷元（Arctigenin）和络石苷元（Trachelogenin）对TPA诱导的小鼠耳肿胀有较强的抑制作用，反映了其在两阶段肿瘤发生过程中的抗肿瘤作用;从络石中提取的黄酮类化合物木犀草素（Luteolin）及其7-O-糖苷（7-O-glycosides）对DPPH自由基有较强的清除活性。(2)从长春花中提取的黄酮类化合物毛蕊花苷能增强TPA抑制的小鼠迟发型超敏反应，表明毛蕊花苷可能增强免疫系统对癌症的抵抗力。(3)长春花中的2，3-二羟基苯甲酸具有较强的DPPH自由基清除活性，这与较低的癌症风险有关，并可防止晚期糖基化终产物（AGEs）的形成，这可能会减少糖尿病并发症的发生。(4)2.抗过敏活性筛选试验（1）车前草中的苯乙醇苷（Plantamajoside）和车前草中的黄酮类化合物黄芩素（Scutellarein）和6-羟基木犀草素（6-hydroxyluteolin）对5-脂氧合酶有抑制作用，与抗过敏有关。黄酮类化合物具有抗肥大细胞组胺释放活性。苯乙醇苷对花生四烯酸诱导的小鼠耳肿胀有抑制作用。(2)连翘苷、连翘酯苷和毛蕊花苷是连翘中的苯乙醇苷类化合物，具有较强的5-脂氧合酶抑制活性和DPPH自由基清除活性，与抗过敏有关。(3)秦皮壳酮是秦皮中的一种香豆素类化合物，对5-脂氧合酶、醛糖还原酶和家兔血小板聚集有较强的抑制作用。秦皮壳还表现出较强的清除DPPH自由基的活性。

1. The screening assay for anticancer activity(1) Arctigenin and trachelogenin, lignans from Caulis Trachelospermi, were shown to have a high inhibitory effect on TPA-induced ear edema, which reflects an antitumor-promoting effect in two-stage carcinogenesis.Luteolin and its 7-O-glycosides, flavonoids from Caulis Trachelospermi, showed a strong DPPH radical-scavenging activity.(2) Mauritianin, a flavonoid from Herba Catharanthi, enhanced the TPA-suppressed delayd type hypersensitivity reaction in mice, indicating that mauritianin may augument the resistance of the immune system to cancer.(3) 2,3-Dihydroxybenzoic acid from Periwinkle showed a strong DPPH radical-scavenging activity, which is associated with a lower risk of cancer, and prevented the formation of advanced glycation end productions (AGEs), which may reduce development of diabetic complications.(4) Syringaresinol-di-glucoside, a lignan from Eleutherococcus, displayd an antigastric ulcer effect in restrained, cold-water-stressed rats.2.The screening assay for anti-allergic activity(1) Plantamajoside, a phenylethanoid glycoside from Plantago Herb, and scutellarein and 6-hydroxyluteolin, flavonoids from Plantago Herb, were shown to have inhibitory activity on 5-lipoxygenase, which has the relationship with anti-allergy. Flavonoids showed anti-histamine release activity from mast cells. Phenylethanoid glycosides showed inhibitory activity on arachidonic acid-induced mouse ear edema.(2) Forsythiaside, suspensaside and acteoside, phenylethanoid glycosides from Forsythiae Fructus, showed a potent inhibitory activity on 5-lipoxygenase and a strong DPPH radical-scavenging activity, which have the relationship with anti-allergy.(3) Esculetin, a coumarin from Cortex Fraxini, showed strong inhibitory activities on 5-lipoxygenase, aldose reductase and rabbit platelet aggregation. Also esculletin showed a strong DPPH radical-scavenging activity.