Pharmacological studies on the effects of kanpo medicine on the injury of rat liver by ischemia-reperfusion.

给10周龄雄性Wistar大鼠灌胃输液100 mg/kg，连续4d，末次给药后1h复制实验性肝损伤模型。肝脏血液再灌流1h后，测定血清中GOT、GPT和LDH。测定肝组织匀浆中的过氧化脂质，并以每毫克蛋白生成的丙二醛为单位表示。测定了总谷胱甘肽含量、细胞色素P-450、细胞色素b5和NADPH细胞色素C还原酶活性。对照组大鼠的过氧化脂质含量为6.82+-0.54，重赛康组大鼠的过氧化脂质含量降至5.11+-0.15。而总谷胱甘肽含量、细胞色素P-450、细胞色素b5和NADPH细胞色素C还原酶活性无明显变化。“Shosaikoto”似乎不能预防肝脏损伤。在上述实验后，大柴胡100、200和400 mg/kg剂量，或DoS…中的Hochuekkito8周龄雄性Wistar大鼠灌胃给予ES 200和400 mg/kg，连续4d，末次给药1h后15min复制中度实验性肝损伤模型。肝脏血液再灌流1h后，测定血清中GOT、GPT和LDH。测定肝组织匀浆中的过氧化脂质，并以每毫克蛋白生成的丙二醛n摩尔表示。对照组大鼠(缺血组)的过氧化脂质含量为7.06±0.67，大豆根200 mg/kg组的过氧化脂质含量为6.58±0.33和6.33±0.55。因此，《大赛本》有减少内容的趋势。而200 mg/kg和400 mg/kg剂量组大鼠体内含量并未降低。治疗后肝损伤轻微缓解，用“大赛本”和“Hochuekito”。上述结果表明，“Shosaikoto”和“Daisaikoto”对肝损伤的缓解作用可能是通过捕获过氧化脂质来实现的。“Hochuekito”可能通过不同的机制发生。较少

"Shosaikoto" in a dose of 100 mg/kg was orally administered for 4 days to 10 week old male Wistar rats and the experimental liver injury model was prepared by 1 hr liver ischemia 1 hr after last administration. After 1 hr blood reperfusion of the liver, GOT,GPT and LDH in the serum were estimated. Lipid peroxides in liver homogenates were measured and expressed as nmol of malonic dialdehyde formed per mg protein. Content of total glutathion, activities of cytochrome P-450, cytochrome b_5 and NADPH cytochrome Creductase were also estimated. The contents of lipid peroxide of control rats were 6.82 <plus-minus>0.54 and those of rats treated with "Shosaikoto" was decreased to 5.11<plus-minus>0.15. However, contents of total glutathion, activities of cytochrome P-450, cytochrome b_5 and NADPH cytochrome C reductase were not altered. "Shosaikoto" dose not seem to protect against liver injury. Following above experiments, "Daisaikoto" in doses of 100,200 and 400 mg/kg, or "Hochuekkito" in dos … More es of 200 and 400 mg/kg were orally administrered for 4 days to 8 week old male Wistar rats and the moderate experimental liver injury model was prepared by 15 min liver ischemia 1 hr after last administration. After 1 hr blood reperfusion of the liver, GOT,GPT and LDH in the serum were estimated. Lipid peroxides in liver homogenates were measured and expressed as n mol of malonic dialdehyde formed per mg protein. Contents of lipid peroxides of control rats (ischemia) was 7.06 <plus-minus>0.67 and those of rats treated with 200 mg/kg of "Daisaikoto" were 6.58<plus-minus>0.33 and 6.33 <plus-minus>0.55, respectively. Therefore, "Daisaikoto" tended to decrease the contents. However, the contents of rats treated with 200 mg/kg and 400 mg/kg of "Hochuekkito" were not decreased. Slight remission of liver injury was observed by treatment with "Daisaikoto" and "Hochuekito". It is concluded that remission of liver injury by "Shosaikoto" and "Daisaikoto" may occur by trapping lipid peroxides. That by "Hochuekito" may occur by different mechanism. Less