Random Amplified Polymorphic DNA (RAPD) Analyzes for Discriminating Genotypes of Microcystis Cyanobactcria and Its Application for the Management of Water Quality of Lakes

应用随机扩增多态性DNA（RAPD）技术对5种蓝藻进行了基因型的区分。具有相同等位酶基因型的各组菌株产生了相似的RAPD图谱，其特征在于各自的组。另一方面，在等位酶基因型不同的菌株之间没有观察到任何相似的RAPD图谱。RAPD分析结果与等位酶分歧值之间的一致性较好，表明这两种方法用于微囊藻属内类群的区分具有较高的可靠性。在RAPD图谱上也观察到了几个扩增的DNA片段，这些片段有望作为具有相同等位酶基因型的特定分类群的标记。RAPD分析和等位酶基因型分析表明，新囊霉、绿霉和韦森霉具有遗传同源性。然而，在铜绿微囊藻和鱼形微囊藻中观察到DNA和蛋白质（等位酶）水平的显著变化。

Random amplified polymorphic DNA (RAPD) analysis was applied to the discrimination of gcnotypes in five species of Microcystis cyanobacteria. Strains of each group possessing the identical allozyme genotype gave similar RAPD patterns characterized by the respective group. On the other hand, any similarities in RAPD patterns were not observed among strains of which allozyme genotypes were different. A good accordance between the RAPD analysis and allozyme divergence indicated a high reliability of the both methods for discrimination of the affiliated group of Microcystis. Several amplified DNA fragments, which were expected as markers for a particular taxon with identical allozyme genotype, were also observed on the RAPD patterns. Genetic homogenieties of M.novacekii, M.viridis and M.wesenbergii were shown by RAPD analysis as well as allozyme genotypes. However, significant variations were observed in M.aeruginosa and M.ichthyoblabe in the levels of DNA and proteins (allozymes).