Studies of cytology, enzyme electrophoresis and genomic in situ hybridization (GISH) on the evolution of Polystichum

对日本蓼的染色体数目、减数分裂行为、基因组原位杂交和酶电泳进行了研究。多面虫（室）ex kunze) Pr.、P.longifrons kurata、p.a tagawanum kurata、p.a pseudomakinoi Tagawa、p.a ovatopaleacum (Kodama) kurata和p.a makinoi (Tagawa) Tagawa为四分体，2n=164。其中许多在I中期显示出82个二价，并有性繁殖。田川蓼（Polystichum kiusiuensis Tagawa）；前库恩（C.Chr）P.otomasui Sa。Kurata和p.b fibrilloso-paleaceum (Kodama) Tagawa为二倍体，2n=82。在减数分裂中期，p.a exium和p.a fibrilloso-paleaceum形成41个二价体。所有的推定杂交品种均为四倍体，2n=164，且减数分裂不规则。所有的推定杂交品种包括：仓田种猪、仓田种猪、仓田种猪、仓田清祖种猪、赤泽种猪和仓田高稻种猪。性四倍体的等位酶变异表现出典型的异源多倍体形态。孢子体杂合子带在配子体中不分离，为固定杂合子带。杂种的杂合性通过其同工酶模式得到证实。四倍体种间的遗传同一性值较高，而二倍体与四倍体种间的遗传同一性值较低。GISH还证实了二倍体和四倍体物种之间的遗传差异。

Chromosome number, meiotic behavior, genomic in site hybridizatio, enzyme electrophoretic studies were made on Japanese Polystichum. Polystichum Polyblepharum (Room. ex kunze) Pr., P.longifrons kurata, P.tagawanum Kurata, P.pseudo-makinoi Tagawa, P.ovatopaleacum (Kodama) Kurata, and P.makinoi (Tagawa) Tagawa were tetrads with 2n=164. Many of them displayd 82 bivalents in metaphase I,and reproduced sexually. Polystichum kiusiuensis Tagawa, P.eximium (Mett.Ex Kuhn) C.Chr. P.otomasui Sa.Kurata and P.fibrilloso-paleaceum (Kodama) Tagawa were diploids with 2n=82. In meiotic metaphase I,41 bivalents were formed in P.eximium and P.fibrilloso-paleaceum. All of putative hybrids P.*anceps Kurata, P.*hakonense kurata, P.*hatajukuense Kurata, P.*kiyozumianum Kurata, P.*pseudo-ovatopaleaceum Akasawa and P.*takaosanense Kurata were tetraploids with 2n=164 and their meiosis were irregular. Allozyme variations of sexual tetraploids displayd typical allopolyploidal patterns. Heterozygotic bands of sporophytes did not segregate in gametophyes, and they were fixed heterozygosity. Hybridities of putative hybrids were confirmed by their allozyme patterns. Genetic identity values between tetraploid species were high, while those values between diploids and tetraploids were low. GISH also confirmed genetic differences between diploid and tetraploid species.