A new method of NMR imaging for the study of drug dynamics in vivo by the use of high field, vertical magnet.

(1)该提议是一种利用易于获得的高分辨率核磁共振磁体在动物体内动态研究药物的新方法的项目。高场磁铁的使用提供了低浓度药物代谢图谱所需的高灵敏度。如果被证明是适用的，大多数机构配备的核磁共振仪器应该被发现对利用动物进行药物的可行性研究是有用的。(2)1996年发展了化学位移选择性同时观测的成像序列。与多核成像探针相结合，该序列已成功地应用于抗癌药物5-FU的动态研究(1997年在温哥华举行的第五届ISMRM上报道)。(3)该序列已被修改，以便于1997年进行高速观察。5-氟尿嘧啶的有效代谢物F-核苷酸的成像是首次报道。(1997年在阿尔伯克基举行的第三届ICMRMM上报道)高速MRI射频加热对小鼠的影响很小。(4)本项目的目的已经成功实现。

(1) The proposal has been the project of a new method for in vivo dynamical study of drugs in animals by the use of an easily accessible, high resolution NMR magnet. The use of high field magnet provides high sensitivity required for the metabolic mapping of drugs in low concentration. If proved to be applicable, NMR instruments equipped in most of the institution should be found to be useful for the feasibility studies of drugs using animals.(2) The imaging sequence for the chemical shift selective, simultaneous observation has been developed in 1996. In combination with the multi-nuclea imaging probe, this sequence has been applied successfully for the dynamical study of anti-cancer drug 5-FU.(Reported at the 5th ISMRM,Vancouver, 1997)(3) The sequence has been modified for high speed observation in 1997. The imaging of the F-nucleotides, the effective metabolites of 5-FU,has been reported for the first time. (Reported at the 3rd ICMRMM,Albuquerque, 1997) The effect of rf heating by the high speed MRI on the mice was found to be small.(4) The purpose of the project has been successively achieved.