Herpesviridae infection in Okinawa : With special reference to infection to nervous system

单纯疱疹病毒：从日本冲绳生殖器疱疹中分离到单纯疱疹病毒2型（HSV 2）10株，其中K1、K2、K3株和标准株SAV经腹腔接种BALB/cN和C57 BL/6 N小鼠。在C57 BL/6 M小鼠中清楚地观察到肌萎缩。在死亡前24小时内，小鼠表现出在C57 BL/6 N小鼠中观察到的尿失禁和直肠失禁。在死亡前24小时内，小鼠表现出尿失禁和直肠失禁以及后腿截瘫。随机分布的，严重的坏死表现在脊髓，主要是在较低的脊髓。在Balb/cN小鼠中，没有明显观察到临床症状，因为小鼠在病毒接种后不久就死亡了。虽然C57 BL/6 N小鼠的脊髓坏死比BALB/cN小鼠更突出，但脑坏死仅在之后发现，而在前者中未发现。然而，在大脑中，通过PCR和免疫组织化学证实了病毒。最弱毒力K3株，不引起脊髓坏死，无动物死亡。人疱疹病毒8型（Kaposi肉瘤相关疱疹病毒，KSHV）：对93例良性和恶性血管病变（包括5例Kaposi肉瘤）进行了PCR分析。5例Kaposi肉瘤和1例化脓性肉芽肿均为KSHV阳性，而其他血管病变、6例梭形细胞血管内皮瘤、1例Kaposi型血管内皮瘤、8例血管肉瘤、46例血管瘤、7例血管球瘤和19例化脓性肉芽肿均为KSHV阴性。应用聚合酶链反应（PCR）和原位杂交技术对口腔鳞癌组织中的EB病毒（EBV）进行检测。EB病毒DNA主要分布在浸润的淋巴细胞上。

Herpes simplex virus : Herpessimplex virus type2 (HSV2) strains (10 strains) were isolated from genital herpes in Okinawa Among them, K1, K2 and K3 strains and standard laboratory strain, SAV,were inoculated intraperitoneally into BALB/cN and C57BL/6N mice. The myelitis was clearly observed in C57BL/6M mice. Within 24 hours before death, the mice showed urinary and rectal incontinence observed in C57BL/6N mice. within 24 hours before death, the mice showed urinary and rectal incontinence and paraplegia of the hind legs. Randomly distributed, severe necrosis was demonstrated in the spinal cord, mainly at the lower cord. In BALB/cN mice the clinical symptoms were not clearly observed, as the mice died shortly after viral inoculation. Although spinal cord necrosis was more prominent in C57BL/6N mice than BALB/cN mice, brain necrosis was only found later, and not in former. In the brain, however, the virus was demonstrated by both PCR and immunohistochemistry. The weakest virulent strain K3, did not cause spinal cord necrosis, and no animals died. Experimental myelitis depended on both viral strain and also mouse strain.Human herpesvirus type 8 (Kaposi sarcoma associated herpesvirus, KSHV) : PCR analysis of 93 cases of benign and malignant vascular lesions including 5 cases of Kaposi sarcoma was performed. All 5 cases of Kaposi sarcoma and one of granuloma pyogenicum were positive for KSHV.But other vascular lesions, 6 spindle cell hemangioendotheliomas, 1 kaposi form hemangioendothelioma, 8 angiosarcomas, 46 hemangiomas, 7 glomus tumors and 19 pyogenic granulomas were negative for KSHV.Sequence analysis of KSHV from Okinawa revealed that it was classified into type C.EBV : PCR and in situ hybridization analysis of EBV on oral squamous cell carcinomas were perfomed. EBV DNA was mainly demonstrated on the infiltrated lymphocytes.