The funcation of parathyroid hormone-related protein.

应用免疫组织化学和原位杂交技术研究42例长期血液透析患者甲状旁腺增生组织中甲状旁腺激素（PTH）及PTH相关蛋白（PTHrP）的定位。结果与PTH/PTHrP免疫染色同一切片的增殖活性（PCNA指数：增殖细胞核抗原阳性细胞数/100个细胞）进行比较。10个正常腺体作为对照。在增生中，免疫组织化学观察到PTH或PTHrP阳性或两者均阳性的细胞。PTHrP mRNA表达区与PTHrP免疫组化阳性区完全吻合。嗜氧细胞或移行嗜氧细胞均呈PTHrP阳性，并呈聚集的趋势。PCNA阳性细胞在正常腺体中少见，其指数为0.22（±±），平均值为0.09（±±）。(SEM)。增生性病例增殖细胞成分显著增加，PTHrP阳性细胞指数为1.25(]SY.+- . [)0.16， PTHrP阴性细胞指数为7.8(]SY.+- .[)0.52。因此，在继发性增生中，PTHrP阳性细胞的增殖活性显著低于PTHrP阴性细胞（P<0.0001）。尽管PTHrP最初被报道为恶性肿瘤中的高钙血症物质，但现在主要怀疑它是调节细胞生长或分化的自分泌或旁分泌物质。在抑制PTHrP阳性细胞增殖的基础上，我们提出PTHrP在人甲状旁腺中作为生长抑制剂的功能作用。

The localization of parathyroid hormone(PTH)and PTH-related protein(PTHrP)were studied in 42 hyperplastic parathyroid glands in long term hemodialysis cases with immunohistochemistry and in situ hybridization. Results were compared with proliferaative activity(PCNA index : counts of proliferating cell nucleaar antaigen-positive cells/100 cells)on the same section used for PTH/PTHrP immunostaining. Ten normal glands weree studied as the control.In hyperplasia, cells positive for either PTH or PTHrP,or both, were observed immunohisstochemically. The areas expressing PTHrP mRNA were completely coincided with those positive for PTHrP immunohistochemically. Oxyphil or transitional oxyphil cells were consistently positive for PTHrP and showed a tendency for grouping together. The PCNA positive cells were rare in normal glands with index of 0.22(]SY.+-。[)0.09(mean (]SY.+-。[) SEM). Proliferating cell components were significantly increased in hyperplastic cases and indices for PTHrP positive cells were 1.25(]SY.+-。[)0.16 and tose for PTHrP negative cells were 7.8(]SY.+-。[)0.52. Thus, the proliferative activity in PTHrP positive cells was sgnificantly loweer than their PTHrP negative conterparts in secondary hyperplasia(P<0.0001).PTHrP is now mostly suspected to act as an autocrine or a paracrine substance regulating cell growth or differentiation, although it was originally reported as the hypercalcemic substance in malignancies. On the basis of suppressed cell proliferation in PTHrP positive cells, we suggest a functional role of PTHrP as growth suppresser in the human parathyroid glands.