A new analytical method using a new reversed-phase chromatographic columnn a 2-mum porous microspherical silica gel.

最近，一种新的反相色谱柱，TSK凝胶Super-ODS，基于2 μ m硅胶，从Toshoh（东京，日本）商业上可获得。该ODS柱比常规ODS柱（粒径5 μ m）具有更好的分离度和更快的分离速度。1995年和1996年分别用该柱同时测定了人血清中抗癫痫药和苯二氮卓类药物，并与常规ODS柱进行了比较。1997年建立了环类抗抑郁药的高效液相色谱法。（1）与传统的柱填料相比，在室温下，即使没有梯度洗脱，也可以预期更快速的测定，（2）所需的有机溶剂量更少，（3）更灵敏的测定是可能的。这些结果表明，粒径为2 μ m的新ODS填料更灵敏，提供了更好的分离，并且比常规ODS填料更快速。

Recently, a new reversed-phase chromatographic column, TSK gel Super-ODS,based on 2-mum silica gel, became commercially available from Toshoh (Tokyo, Japan). This ODS colum achieves better resolution and fast separation than that of conventional ODS column (particle size 5mum). We report the simultaneous determinaition of antiepireptic drugs and benzodiazepine drugs in human serum using this reversed-phase column in comparison with the conventional ODS column in 1995 and 1996, respectively.A high-performance liquid chromatographic method has been developed for the forensic analysis of cyclic antidepressant drugs in 1997.This ODS column packing has the following advantages : (1) more rapid determination can be expected at room temperature, even without gradient elution compared with the conventional column packing, (2) the amount of organic solvent required is smaller and (3) more sensitive determination is possible.These results show that a new ODS filler with a particle size of 2mum was more sensitive, provided better separation, and was more rapid than with conventional ODS filler.