Mechanisms for premenstrual exacerbation of asthama

将卵黄蛋白联合氢氧化铝皮下注射和百日咳疫苗腹腔注射分别致敏16只雌性雌性褐挪威大鼠。致敏后，切除雌性大鼠卵巢，按前文描述分为4组（每组n=4）。第一组为去卵巢大鼠，分别在去卵巢后第3、4、7、8天注射雌二醇，在去卵巢后第4、8天（平均基本周期长度4.1天）注射黄体酮。第2组在第3、4、7、8天只给予雌二醇治疗，第3组在第4、8天只给予孕酮治疗，第4组不给予类固醇治疗。在第8天，雌二醇和黄体酮治疗在抗原激发开始前4小时进行。卵巢切除术后8天，所有组均雾化卵清蛋白，并在机械通气下监测气道内压力，以检测抗原暴露后的即时和晚期气道反应。支气管肺泡灌洗（BAL）和组织学检查评估抗原暴露后8小时气道炎症。在接受周期性雌激素和孕酮替代的组中，与其他组相比，即时和晚期气道反应的幅度均显着降低，并且攻毒后8小时BAL液中恢复的中性粒细胞数量显着低于其他组。我们的结论是，孕酮和雌激素的周期性管理抑制早期和晚期的反应吸入过敏原雌性褐挪威大鼠。

Sixteen female virgin Brown Norway Rats were sensitized with ovalubumin in combination with Aluminium hydroxide injected subcutaneously and Bordetera Pertussis vaccine intraperitoneally. After sensitization was performed, female rats were ovariectomized and divided into four groups (n=4 each) as described previously. The first group consisted of ovariectomized rats which received estradiol at 3,4,7 and 8 days after ovariectomy, and progesterone at 4 and 8 days cyclically (mean basic cycle length is 4.1 day) after ovariectomy. The second group was treated only with estradiol at 3,4,7 and 8 days, the third group was treated only with progesterone at 4 and 8 days, while the fourth group received no steroid treatment. At 8 days, estradiol and progesterone treatment was done at 4 hours before the start of the antigen challenge. Eight days after ovariectomy, all groups were challenged with aerozolized ovalubumin and intra-airway pressures were monitored under mechanical ventilation to detect immediate and late airway responses followed by antigen exposure. Broncho-alveolar lavage (BAL) and histological examination were performed to evaluate the airway inflammation associated with antigen exposure eight hours after challenge. In the group received cyclical replacement of estrogen and progesterone, the magnitude of both immediate and late airway responses was significantly lowered compared to the other groups, and the number of neutrophils recovered in the BAL fluid eitht hours after challenge was significantly lowered compared to other groups. We conclude that cyclical administration of progesterone and estrogen inhibits the early-and late- phase responses to inhaled allergen in female sensitized Brown Norway Rat.