A study of 5-FU metabolism in fatty liver and hepatocellular carcinoma induced by choline deficient diet, using 19F-MRS.

目的：用19F-MRS和31P-MRS评价脂肪肝，分别观察5-氟尿嘧啶代谢（合成为氟核苷酸（Fnct）和分解为α -氟- β -丙氨酸（FBAL））和果糖代谢（主要转化为果糖-1-磷酸）。材料与方法：雄性Wistar Slc大鼠经BEM170/200 （4.7T，大冢电子）表面线圈直径17mm检测。胆碱缺乏日粮（CD日粮）给药2、4周诱导脂肪肝。静脉注射5-FU后100min，获得19F-MR光谱。获得果糖注射前后的31P-MR光谱。19F-MRS和31P-MRS分别进行实验。结果：CD饮食组在19F-MRS中Fnct峰高，FBAL峰受抑制。在31P-MRS中，CD饲粮组与对照饲粮组在磷酸单酯（PME）和三磷酸腺苷（ATP）的时间过程上无显著差异。结论：我们检测到Fnct的峰值可能反映了肝损伤后的恢复反应，但果糖代谢差异不明显。在受损肝脏中，不同疾病和个体的代谢途径和严重程度不同。活体磁共振动态研究有可能检测损伤点和严重程度。我们建议尝试不同的方法来评估肝功能是非常重要的，体内动态磁共振可能是有价值的。

Purpose : To assess fatty liver by 19F-MRS and 31P-MRS,observing 5-fluorouracil metabolism (anabolize to fluoronucleotide (Fnct) and catabolize to alpha-fluoro-beta-alanine (FBAL)) and fructose metabolism (mainly inverted to fructose-1-phosphate), respectively.Materials and Methods : Male Wistar Slc rats were examined on BEM170/200 (4.7T,Otsuka Electronics) with 17mm-diameter surface coil. Fatty liver was induced by choline deficient diet (CD diet) given for 2 or 4 weeks. 19F-MR spectra were obtained for 100min.after 5-FU intravenous injection. 31P-MR spectra were obtained pre and post fructose injection. 19F-MRS and 31P-MRS studies were performed in separated experiments.Results : In 19F-MRS,high Fnct peak and suppressed FBAL peak was observed in CD diet group. In 31P-MRS,no significant difference could be seen between CD diet group and control diet group about the time course of phosphomonoester (PME) and adenosine-triphosphate (ATP).Conclusion : We detected the high peak of Fnct that may reflect the recovering reaction from liver damage but the difference of fructose metabolism was not evident. In damaged liver, the injured metabolic pathway and severity is different among the diseases and individuals. The dynamic study with in vivo MRS has the possibility to detect the impaired point and the severity. We proposed that it is very important to try some different methods to evaluate liver function and in vivo dynamic MRS may be valuable for it.