Efficary of the thermo-chemotherapy for experimental peritoneal carcinomatosis in the rabbits.

本研究采用1 × 10^6 ~（-2）细胞腹腔注射法建立兔腹膜癌转移模型，观察热化疗对兔腹膜癌转移的作用机制。三周后，在麻醉下对兔进行剖腹手术，并使用以下试剂进行30分钟的连续腹膜冲洗;例如，A组为38 ℃生理盐水组，B组为43 ℃热盐水组，C组为38 ℃生理盐水加顺铂20 mg组，D组为43 ℃生理盐水加顺铂20 mg组。1）临床疗效的大体评估结果是A组（n=6）中所有PD，B组15个中11个PD和4个NC，C组10例中PD 4例，NC 6例; D组11例中NC 8例，PR 3例。2)BrdU L.I.为12.6（±）SY。[)7.6 A组为13.8 ± 0.05 ± 0.05。[)4.8 B组为15.2 ± 0.05（± 0.05）μ g/ml。[)5.0 C组为20.6（± SD）。[)4.0在D组。3)Ki-67淋巴细胞指数为29.3 ± 0.01。[)6.2 A组为32.0 ± 0.05 ± 0.05。[)7.6 B组为29.2 ± 0.05（± 0.05）SY. [)3.7 C组为42.8 ± 0.05（P < 0.01），C组为42.8 ± 0.05（P < 0.01）。[)5.2在D组。4)TUNEL法检测细胞凋亡的表达。细胞凋亡指数为3.8（± SD）。[)2.8 A组为11.0 ± 1.0 SY。[)6.3 B组为18.6 ± 0.18（SY. ±. [)9.8 C组为38.0 ± 0.05，P < 0.01。D组29.4。5)D组的Pt血清浓度水平较高，而C组的Pt组织浓度水平较高。6)结论：D组肿瘤细胞增殖率增高，同时凋亡率增高，可能是热化疗对肿瘤细胞周期的影响，从而导致肿瘤细胞凋亡。

Using a newly established rabbit model of peritoneal carcinomatosis, the mechanism of the effect of thermo-chemotherapy against this pathological status was investigated.To make the peritoneal carcinomatosis in a rabbit, 1*10^6 Vx2 cells were injected intra-peritoneally. Three weeks afterward, the rabbit was laparotomized under anesthesia and 30 minutes of contineous peritoneal irrigation was conducted using the following agents ; e.g., the group A using normal saline of 38ﾟC,the group B using heated saline of 43ﾟC,the group C using 38ﾟC saline with 20 mg of CDDP and the group D using 43ﾟC saline with 20 mg of CDDP.Results : 1) Gross assessment of clinical efficacy resulted PDs in all of the A Group (n=6), 11 PDs and 4 NCs out of 15 in the B group, 4 PDs and 6 NCs out of 10 in the C group and 8 NCs and 3 PRs out of 11 in the D group. 2) BrdU L.I.was 12.6(]SY.+-。[)7.6 in the A group, 13.8(]SY.+-。[)4.8 in the B group, 15.2(]SY.+-。[)5.0 in the C group and 20.6(]SY.+-。[)4.0 in the D group. 3) Ki-67 L.I.was 29.3(]SY.+-。[)6.2 in the A group, 32.0(]SY.+-。[)7.6 in the B group, 29.2(]SY.+-。[)3.7 in the C group and 42.8(]SY.+-。[)5.2 in the D group. 4) Expression of the apoptosis was examnined by means of TUNEL methods. A poptotic index was 3.8(]SY.+-。[)2.8 in the A group, 11.0(]SY.+-。[)6.3 in the B group, 18.6(]SY.+-。[)9.8 in the C group and 38.0(]SY.+-。[)29.4 in the D group. 5) The serum concentration level of Pt was higher in the D group whereas the tissue concentration level of Pt was higher in the C group. 6) The serum creatinin, BUN and potassium were higher in both C and D group.Conclusion : The ratio of the proliferative tumor cells was increased and, at the same time, apoptosis was highly observed in the D group that might imply the affect of thermo-chemotherapy upon tumor cell cycle induced the apoptosis.