子宮内膜癌抑制遺伝子の細胞老化活性を指標としたクローニング

为了明确人类1号染色体上参与人子宫内膜癌发生的衰老（抑制）基因位点（HC-1），我们引入了两种q臂缺失的HC-1，一种（del1-q）在q12-q31之间有间质缺失，另一种（GM1173）在q臂几乎完全缺失。Del-1q表现出诱导理科的活性，而GM1173则没有。这些结果表明基因位点携带在1q31上，一些亚染色体可转移片段（STFs）定位到q31的特定区域，进入HHUA细胞。窝藏q31-41的STF具有该活动。根据LOH研究，通过STFs转移所确定区域的连续STS标记分析癌症标本，衰老基因位于1q41-42上标记D1S225和D1S459两侧的区域。

In order to define the senescence (suppressor) gene locus involved in human endometriaI carcinogenesis on human chromosome 1 (HC-1), we introduced two kinds of the HC-1 which has deletions on q arm, one (del1-q) has interstitial deletion between q12-q31 and the other (GM1173) has almost complete deletion of q arm. Del-1q showed senscence-inducing activity but GM1173 did not. This result indicated the gene locus is carried on 1q31 to tel. Several subcromosomal transfferable fragments (STFs) addressed to certain regions of q31 to tel into HHUA cells. A STF harboring q31-41 had the activity. Following LOH study analyzing the cancer specimens by contiguous STS markers in the region determined by the STFs transfer suggested that the senescence gene locates within the region flanked by marker D1S225 and D1S459, on 1q41-42.