Immunohistochemical study on cellular proliferative marker and genetic alteration

本文对舌鳞癌（SCC）细胞增殖标志物PCNA、NORs、p53、Cyclin D1、p34cdc2、bcl-2基因表达及凋亡与肿瘤侵袭方式的关系进行了一系列研究。本项目使用了67个舌SCCs标本。采用免疫组化染色法检测PCNA及遗传蛋白表达，采用siverI染色法检测NORs。通过显微镜下计算免疫染色肿瘤细胞的阳性率来评价这些标志物。采用Yamanoto - Kohama法确定肿瘤的侵袭模式。结果1)PCNA在1-3型肿瘤中表达阳性率较低（26.5*7.9），在4型肿瘤中表达阳性率较高（41.1 * 6.7）。2) 1-3模式肿瘤的NORs数量较低（3.2*0.8），而4.3模式肿瘤的NORs数量较高。在1-3型肿瘤中表达量较低（10/24,41.3%），在4型肿瘤中表达量较高（12/19,92.3%）。4) p53在16134例1-3型肿瘤中频率较高（47.0%），在9/13例4型肿瘤中频率较高（69.2%）。5)p34cdc2在24/48例1-3型肿瘤中频率较高（50.0%），在10/15例4型肿瘤中频率稍高（62.7%）。6) 1-2模式肿瘤细胞凋亡标记值为2.53 *1.0,3模式肿瘤细胞凋亡标记值为1.95*1.5,4.7模式肿瘤细胞凋亡标记值为1.5 * 1.04。1-2模式肿瘤细胞中有4/13（30.8%）表达bcl-2, 3模式肿瘤细胞中有11/27（40.07%）表达bcl-2, 4模式肿瘤细胞中有9/11（81.8%）表达bcl-2。结论舌鳞状细胞癌中PCNA、NORs、Cyclin D1、p53、p34cdc2、bcl-2的高表达在高侵袭性肿瘤中更为常见。相反，在这些肿瘤中，凋亡的标记值相对较低。这些结果提示与细胞增殖活性相关的基因异常参与肿瘤侵袭。少

A series of studies were performed on the relations of cellular proliferative markers of PCNA, NORs, the genetic expressions of p53, Cyclin D1, p34cdc2, bcl-2 and apoptosis in tongue squamous cell carcinoma (SCC) with the mode of tumor invasion. The specimens from 67 tongue SCCs were used for this project. Immunohistochemical staining was employed to detect PCNA and the expression of genetic proteitns, and siverI staining method employed to examine NORs. The evaluation on those markers was performed by the positive rate of immunostained tumor cells calculated under microscopic field. The mode of tumor invasion was determoned by the method of Yamanoto and Kohama.Results1) PCNA was expressed with a lower positive rate (26.5*7.9) in tumors of mode 1-3, and with a high positive rate ( 41.1 * 6.7) in tumors of mode 4. 2) The numbers of NORs was found with a lower value (3.2*0.8) in tumors of mode 1-3, and with a relatively high value in tumors of mode 4.3) Cyclin Dl. was, with a relatively … More lower level, expressed in tumors with mode 1-3 (10/24, 41.3%), and with a high level in tumors with mode 4 (12/19, 92.3%).4) p53 expression was detected with a frequency in 16134 tumors with mode 1-3 (47.0%), and a relatively high frequecy in 9/13 tumors with mode 4 (69.2%).5) p34cdc2 expression was detected in 24/48 tumors with mode 1-3 (50.0%), and comparatively with a little high frequency in 10/15 tumors with mode 4 (62.7%).6) The apoptosis was labelled with the value of 2.53 *1.0 in tumors with mode 1-2, with the value of 1.95*1.5 in tumors with mode 3 and with the value of 1.5 * 1.04 in tumors with mode 4.7) bcl-2 expression was detected in 4/13 tumors with mode 1-2 (30.8%), in 11/27 tumors with mode 3 (40.07%) and in 9/11 tumors with mode 4 (81.8%).Conclusionln tongue SCC, the high level expressions of PCNA, NORs, Cyclin D1, p53, p34cdc2 and bcl-2 were more frequently detected in high invasive tumors. Conversely, apoptosis was labelled with relatively lower value in those tumors. These resuIlts suggest that the genetic abnormalities in relation to cellular proliferative activity involve in tumor invasion. Less