The Histological Study of Ridge-augmentation by Using Guided Tissue Regeneration

引导性骨再生（Guided Bone Regeneration， GBR）是一种有效的修复骨脊的方法，但引导性骨再生所使用的材料不具有可吸收性，存在膜暴露感染和二次手术切除的问题。因此，本研究的目的是通过研制已成型的可吸收膜，并将其应用于犬骨缺损模型，探讨骨缺损中嵴增强的可能性。双侧下颌第一磨牙于3个月前拔除，本研究开始。切开无牙部分牙龈，将颊黏骨膜瓣向舌侧提起露出骨后，取出5mm*5mm的颊、舌骨壁段，形成骨缺损，连续颊-舌。实验组采用棒状增强胶原膜包覆骨缺损，可获得约5mm高的间隙，对照组仅重新定位龈瓣缝线。术后即刻及术后12周采用单独制作的咬合块和长锥技术在固定摄影条件下获得更多根尖周围x线片。术后12周对x线片进行减影分析。按普遍规律制作中、远端连续切片，染色进行组织病理学检查。x线片上，对照组对实验部位牙槽骨周长的变异认识较多，实验组对实验部位的再生体积的变异认识较多。对照组多见凹形牙槽骨，试验组在组织病理学上见原牙槽骨嵴移位扁平的骨再生。新生骨与原牙槽骨的边界部分相对模糊。实验组胶原膜残余几乎不可见，成膜部位周缘未见炎性细胞。少

Guided Bone Regeneration (GBR) is demenstrated that is useful in ridge augmentation, but so that materials which is used with GBR is non-absorbable, those problems of infection with membrane exposure and the second operation of materials removal had been indicated.So the purpose of this study is to examine possibility of ridge augmentation in bone defects, with we develop absorbable membrane having take form, and using it in dog bone defect model.Bilateral mandibular first molars had been extracted 3 months previously and this study was started. After edentulous part gingiva was incised and buccal mucoperiosteal flaps were raised to ward the lingual side to expose bone, buccal and lingual bone wall segments measuring 5mm*5mm were removed to form bone defects, continuous bucco-lingually.The experimental group coated bone defects by rod reinforcement collagen membrane as can get space of approximately 5 mm high and the control group did only reposition suture of gingival flap.Periapical … More radiographs were obtained with individually manufactered bite blocks and the long-cone technique and fixed photography condition in right after operation and after an operation 12 weeks. There in right after operation/after an operation 12 weeks radiographs were evaluated analysis by Subtraction.We made mesiodistal serial sections according to universal law and there were stained for histopathological examination.The variate of circumference original alveoler bone of experiment parts was recognized much in control group and regeneration volume in experiment parts was recognized much in experimental group for radiographs.Concavity recognized alveoler bone much in control group and bone regeneration that original alveoler bone crest was shifted to flatly was recognized in experimental group for histopathological findings.boundary part of neogenesis bone and original alveoler bone was indistinctness relatively.The remain of collagen membrane was hardly recognized and the inflammatory cells wasn't observed in circumference of membrane setting part in experimental group. Less