Construction of periodontal ligament tissue around artificial dental roots

1.牙周膜细胞微载体三维培养成为可能。明确了适宜的培养条件.牙周膜上皮细胞表达碱性磷酸酶活性在体外，而不是在体内。提示牙周上皮细胞在培养环境中发生了转化. PDL细胞通过同时表达ODF和OCIF mRNA来调控破骨细胞的生成.牙周组织中胶原αI（1）mRNA水平的降低和胶原αI（1）基因启动子区DNA甲基化与牙周组织的年龄依赖性退行性改变有关.与牙龈成纤维细胞相比，人牙周膜细胞表达CD14 mRNA和膜CD14的水平较低，对LPS的反应也较低.结果表明，牙周上皮细胞在脱细胞牙骨质形成过程中起着向成牙骨质细胞分化的作用。细胞牙骨质区的PE则参与了牙本质细胞间质的相互作用， ...更多信息 牙骨质破坏，TUNEL阳性反应.通过观察牙窗缺损通过骨延伸到根面的愈合过程，提示牙周膜只能从原来健康的牙周膜再生，牙骨质的再生过程类似于骨的改建.种植体表面可见牙骨质和牙周韧带样组织。另一方面，在嵌入牙槽骨的种植体上观察到骨。结果表明，牙周膜细胞种植于牙本质腔内可促进牙骨质的形成。免疫组织化学方法仅在种植腔中检测到BrdU标记的PDL细胞。将培养的牙周膜细胞植入牙本质腔内，观察到牙周膜样组织和新形成的牙骨质样组织。结果表明，对切牙和磨牙敏感的牙周膜机械敏感神经元在初级和次级躯体感觉皮层的不同区域投射。少

1. The three-dimensional cell culture became possible of periodontal ligament cells with microcarrier. The adequate condition of the culture was clarified.2. Periodontal epithelial cells expressed alkaline phosphatase activity in vitro, not in vivo. It suggests that periodontal epithelial cells transformed in the culture environment.3. PDL cells controled osteoclastogenesis in vitro by express both ODF and OCIF mRNA.4. Decreasing of collagen αI(1) mRNA level and DNA methylation in the collagen αI(1) gene promotor region related to age-dependent degenerative alteration in periodontal tissue.5. Human periodontal ligament cells expressed CD14 mRNA and membrane CD14 at low level as compared with human gingival fibroblasts, and exhibited an only low response to LPS.6. It was suggested that periodontal epithelium (PE) had a role of the differentiation of cementoblasts during the acellular cementum formation. On the other hand, the PE in cellular cementum region was took part in the intermedi … More ate cementum and indicted the positive reaction of TUNEL.7. From the observation of healing process of the fenestration defect through the bone extended into the root surface, it was suggested that periodontal ligament could regenerate only from the original healthy periodontal ligament and the regeneration process of cementum was similar to the remodeling of bone.8. The cementum- and periodontal ligament-like tissue was observed on the titanium implants embedded in the cementum and periodontal ligament. On the other hand, bone was observed on the implants embedded in the alveolar bone.9. It was suggested that the cell-seeding of PDL cells into the intradentinal cavities enhanced the isolated cementum formation. The BrdU marked PDL cells were detected only in the seeded cavities by immunohistochemical method.10. The periodontal ligament-like tissue with newly formed cementum-like tissue was observed on the titanium embedded into the intradentinal cavities with cultured periodontal ligament cells in dogs.11. It is suggested that periodontal mechanosensitive neurons which were sensitive to the incisor and molar teeth project topographically to the different areas of the primary and secondary somatosensory cortex. Less