Research of Chemiluminescence and Bioluminescence intend to create new functional System

1-1：为了制备高效的化学发光咪唑体系，我们尝试在rubrene的第2位引入咪唑环，由于rubrene部分的能量受体-发射器具有高效的荧光和低的发射能量，这可能有助于获得非常高效的化学发光化合物。1-2：双平面化合物（芳香化合物的4π+4π环加合物）的绝热光环还原是一种激发态，因为反应的效率可以通过光产物的发射来监测（光化学发光，PCL），并讨论了控制激发产物形成效率的因素和激发态势面形状。1-3：化学发光化合物2-甲基-6-（4-甲氧基酚）咪唑[1,2 -α]吡嗪-3(7H)- 1 （MCLA）通过一个单间隔形成酰胺键与环麦芽糖低聚糖（环糊精）结合。在pH为8.0的磷酸盐缓冲液中，研究了合成的环糊精结合MCLA的氧致化学发光性能。发光效率与结合环糊精的种类、MCLA与环糊精之间的间隔长度以及环糊精的结合位点有显著的关系。环甘露糖（γ-环糊精）结合的化合物的发光效率高于环甘露糖己糖或环甘露糖庚糖结合的化合物。2：以2-氨基吡嗪为原料，通过两个区域选择性pd介导的交叉偶联，通过8步合成了dl-Cypridina luciferin及其类似物，并比较了它们的化学和生物发光活性。3：我们分散了大约100个硫脲衍生物，以开发一种灵敏的鲁米诺衍生物化学发光检测方法。提出的鲁米诺及其衍生物的化学发光反应应用于氨基化合物的高效液相色谱分析。

1-1 : In order to prepare a high efficient chemiluminescent imidazole system, we tried to introduce an imidazole ring into the second position of rubrene which may help it to gain a very efficient chemiluminescent compound because of efficient fluorescence and low emission energy in an energy acceptor-emitter of the rubrene moiety.1-2 : Adiabatic photocycloreversion of biplanemers (4π+4π cycloadducts of aromatic compounds) is an excited state because the efficiency of the reaction can be monitored by the emission from the photoproduct (photo-chemiluminescence, PCL), and factors controlling the efficiency for excited product formation and shapes of potential surface of an excited state have been discussed.1-3 : The chemiluminescence compound 2-methyl-6-(4-methoxyphenol)imidazo[1, 2-α]pyrazin-3(7H)-one (MCLA) was attached to cyclomaltooligosaccharides (cyclodextrins) through a single spacer by the formation of an amide bond. The properties of oxygen-induced chemiluminescence of the synthesized cyclodextrin-bound MCLA were investigated in an aqueous phosphate buffer, pH 8.0. The light-emitting efficiency was remarkably dependent on the kind of bound cyclodextrin, spacer length between the MCLA and cyclodextrin, and the binding site in cyclodextrin. The light-emitting efficiencies of cyclomaltooctaose (γ-cyclodextrin)-bound compounds were higher than those of cyclomaltohexaose- or cyclomaltoheptaose-bound compounds.2 : dl-Cypridina luciferin and its analogs were synthesized from 2-aminopyrazine by an eight-step method that included two regio-selective Pd-mediated cross couplings, and their chemi- and bioluminescent actives, were compared.3 : We have scattered about 100 thiourea derivatives in order to develop a sensitive chemiluminescence detection for luminol derivatives. The proposed novel chemiluminescence reaction for luminol and luminol derivatives was applied to a high performance liquid chromatography (HPLC) assay for amino compounds.