Polygyny in the ant Amblyopone silvestrii

本研究的目的是建立一种任意引物PCR DNA杂交的方法，为进一步研究原始的一夫多妻制蚂蚁Amblyopone sivlestrii的社会结构奠定基础。a . silvestrii是一种小蚂蚁，其单个工蚁产生了c.400-700ng的基因组DNA。以该DNA为模板，使用以下合成低聚物作为PCR引物设计锚定微卫星位点的3‘或5’端：1)CAP-PCR的简并引物（3'锚定引物），即（CA）7或（AC）7- dg或DA或DT或DC，以及（GA）7或（AG）7- hg或HA或HT或HC。2) (GA)7ACTG, (GA)7ACTA, (GA)7ACTT, (GA)7ACTC, (AG)7CGTG, (AG)7CGTA, (AG)7CGTT, (AG)7CGTC。3)与前者相同的重复序列，但锚定在3'端，具有2个任意核苷酸残基。4) GA或GT的6个重复固定在5'端，有3到5个任意核苷酸残基。5)无锚点序列（CAG）5、（TAG）5、（GATA）4、（GCGT）4等重复序列。在这些引物中，第2类引物中有1条引物成功地产生了马尾草个体间的多态性。同一类别的另一引物仅在与re -消化结合时也检测到个体差异。虽然这些引物对分析该蚁的亲缘结构，特别是以一夫多妻制为中心的亲缘结构是有用的，但我们还需要进一步的引物。

The aim of this study is to establish the method of DNA flngerprin6g using PCR with arbitrary primers, which is then to be used for the future study on the social structure of the primitive, polygynous ant, Amblyopone sivlestrii. A. silvestrii is a small ant, whose single workers yielded c.400-700ng of genomic DNA. With this DNA as a template, the following synthetic oligomers were used as PCR primers designed to anchor 3' or 5' end of microsatellite loci: 1) Degenerate primers for CAP-PCR (3'-anchord primers), I.e., (CA)7 or (AC)7-DG or DA or DT or DC, and (GA)7 or (AG)7-HG or HA or HT or HC. 2) (GA)7ACTG, (GA)7ACTA, (GA)7ACTT, (GA)7ACTC, (AG)7CGTG, (AG)7CGTA, (AG)7CGTT, and (AG)7CGTC. 3) The same repeats as the former but anchored at 3' end with 2 arbitrary nucleotide residues. 4) 6 repeats of GA or GT anchored at 5' end with 3 to 5 arbitrary nucleotide residues. 5) Repeats without anchor sequence such as (CAG)5, (TAG)5, (GATA)4 and (GCGT)4.Among these primers, one primer of the above category 2 has successfully produced polymorphism between individuals of A. silvestrii. Another primer of the same category also detected individual difference only when combined with RE-digestion. Although these primers are useful for the analysis of kin-structure, especially centered on polygyny, of this ant, yet we need some further primers.