Physiogenetical studies on effect of high environmental temperature on Japanese quail (Coturnix japonica)

本研究的结果可以总结如下：1。与鸡相比，日本鹌鹑的耐热性非常高。临界温度是40度左右的环境温度，成年雌虫表现出接下来的生理变化。(1)在38度的温度下，硬鱼保持产卵，卵巢和肝脏几乎没有变化。(2)在40度时，观察到产蛋停止，肝脏中糖的消耗和脂肪成分的停滞。但是个体的一部分恢复了产卵。(3)在42度时，产蛋完全停止，卵巢和肝脏受到惊吓，糖的消耗进一步进行，肝脏中的脂肪成分完全停止。肝细胞的塌陷也被观察到。日本鹌鹑hsp70至少由CJHSP70A和CJHSP70B两个基因座组成。CJHSP70A的特点如下：这种基因在肝脏、肾脏和睾丸中表达。(2)该基因的氨基酸序列与鸡热休克反应98%的HSP70基因J02579相对应。(3)在蛋白质结合区（PBR）内观察到不影响氨基酸序列的碱基取代，由于限制性内切酶的识别位点：Cfo I和HindIII，这些突变可以很容易地被PCR-RFLPs区分出来。至少存在4个等位基因：pQAR22、pQBR24、pQGF29和pQYR26。另一方面，CJHSP70B的特点如下：(4)该基因至少在肝脏中表达。(5)该基因的氨基酸序列与鸡HSP70基因的AJ004940对应，该基因在非应激99%时表达。(6)根据限制性内切酶识别位点HindIII的突变，至少存在两个等位基因：pQLZ011和pQLZ131。当一个机会序明确了与家禽耐热性有关的生理学时，这一知识是非常重要的，它有助于在高温环境下提高家禽的生产力。少

Results of this research ca be summarized as follows.1. Japanese quail has high heat tolerance very much in comparison with chicken. The critical temperature is an environment temperature about 40 degrees, and physiological changes to show next are observed with adult female. (1) At 38 degrees, bards maintain the laying, and a change is hardly observed in the ovary and the liver. (2) At 40 degrees, the stop of the laying, the exhaustion of the sugar and stagnation of the fat composition in the liver is observed. But a part of the individual resumes the laying. (3) At 42 degrees, the laying stops completely, and an ovary and a liver are daunted, and the exhaustion of the sugar progresses further, and fat composition stops completely in the liver. The collapses of the liver cells are observed also.2. HSP 70 of Japanese quail is composed by at least two loci : CJHSP70A and CJHSP70B.The characteristics of CJHSP70A are as follows. (l) This gene expresses it in a liver, a kidney and a testis … More . (2) The amino acid sequence of this gene corresponds to the J02579 of chicken HSP70 gene that has the heat shock response 98%. (3) The base substitutions that do not influence amino acid sequence inside the protein binding region (PBR) are observed and these mutation can be distinguished easily by the PCR-RFLPs due to the recognition sites of restriction enzymes : Cfo I and HindIII.At least four allelic genes : pQAR22, pQBR24, pQGF29 and pQYR26 exist. On the other hand, the characteristics of CJHSP70B are as follows. (4) This gene is at least expressed in the liver. (5) The amino acid sequence of this gene corresponds to the AJ004940 of chicken HSP70 gene that expressed at the non-stress 99%. (6) At least two allelic genes : pQLZ011 and pQLZ131 based on the mutation of the recognition site of the restriction enzyme : HindIII exist.When an opportunity preface is made clear like the physiology concerned with heat resistance of the domestic fowl, this knowledge is very important, and it can contribute to the productivity improvement of the domestic fowl under the high temperature environment. Less