Utilization of biomass resources-environmental clean up with plant proteins and microorganisms-

Plant growth stimulation with soybean proteins / Microorganisms having potential for soybeandegradation within 48h were isolated. These bacteria were identified as Bacillus circulans，B.stearothermophilus, Streptomyces sp.，degradation products （DSP）有植物生长刺激工厂其中，almost equal level as chemically synthesized fertilizer. The DSP production process with2001 jar fermentor was established.<< plant growth stimulation factors>>The plant growthDSP were analyzed. First of all, stimulation factorssoybean proteins and the degradation products with HCl for plant growth. However，这些产品没有活动。植物组活动也没有检测soybeanproteins degraded products using several proteases.这些结果indicate that plant growthstimulation factors originally from soybean degradation products with HA12, HA19，and MF20. The plant growth stimulation factor from HA12 was isolated as single peak with reversephase chromatography.<<Quantification of microorganisms using nucleic acids from soil>>Time DSP fromthis research also activate microorganisms in soils. Establishment of quantification ofmicroorganisms使用nucleic acids from soils was tried. Efficient nucleic acids isolation方法from soils was investigated. As a result，nucleic acids and microorganisms was determined，and a new quantification of soil microorganisms method was established with nucleic acids fromsoils。

<<Plant growth stimulation with soybean proteins>>・ Microorganisms having potential for soybean degradation within 48 h were isolated.・ These bacteria were identified as Bacillus circulans, B.stearothermophilus, Streptomyces sp., respectively.・ The degradation products (DSP) have plant growth stimulation factors, which were almost equal level as chemically synthesized fertilizer.・ The DSP production process with 2001 jar fermentor was established.<<Analysis of plant growth stimulation factors>>The plant growth stimulation factors from DSP were analyzed. First of all, soybean proteins and the degradation products with HCl were analyzed for plant growth. However, these products have no activity. The plant growth activity was also not detected with soybean proteins degraded products using several proteases. These results indicate that plant growth stimulation factors were originally from soybean degradation products with HA12, HA19, and MF20. The plant growth stimulation factor from HA12 was isolated as single peak with reverse phase chromatography.<<Quantification of microorganisms using nucleic acids from soil>>Time DSP from this research also activate microorganisms in soils. Establishment of quantification of microorganisms using nucleic acids from soils was tried. Efficient nucleic acids isolation method from soils was investigated. As a result, the relationship between quantification of nucleic acids and microorganisms was determined, and a new quantification of soil microorganisms method was established with nucleic acids from soils.