Faihne of hepatocyte growth factor signal transmisson in the hepatic faihne after hepatectomy

实验于去年进行。采用硫代乙酰胺200 mg/kg，每周3次，连续5周给大鼠灌胃造成慢性肝损伤。对这些慢性肝损伤大鼠再切除84%的肝脏制作术后肝功能衰竭模型，设假手术组为对照组，按Day等人的方法对残肝进行MAP激酶活性测定，肝衰竭模型组MAP激酶活性似有所升高，但与对照组相比差异无统计学意义。我们认为，在进一步研究肝衰竭大鼠MAP激酶水平未见明显变化的情况下，进一步研究是否存在下游信号传递失败是不合适的。[HO信号传递失败]另一项研究试图评估是否存在影响细胞流动性的HO下游信号传递失败。然而，这一结果与肝衰竭大鼠MAP激酶的结果并不显著。[残肝组织学检查]取肝切除大鼠，取残肝进行光镜检查。对照组为无肝损伤大鼠，肝切除84%。48h后有丝分裂指数。肝切除组与对照组比较差异无统计学意义。因此，肝切除后发生肝功能衰竭的大鼠可能没有明显抑制肝再生。在此基础上，对前人的研究成果进行了评价和分析。

The investigation was performed following last year.The rat was administrated intraperitoneally thioacetoamide 200mg/kg three times a week for 5 weeks to get chronic liver damage. Postoperative liver failure model was made by additional 84% hepatectomy to these chronically damaged rats Sham operation group was set as control.[MAP kinase activity] MAP kinase assay of the remnant liver was done in the same manner of Day et al, MAP kinase activity was seemed to be up in the hepatic failure model, however, no significant difference was seen compared with the control group. We judged that no appropriatensess was recognized in further investigation to examine the failure of lower stream of the signal transmission, because of the result that no tendency was seen in the level of MAP kinase of the hepatic failure rats.[Failure of signal transmission through ho] Another investigation was tried to evaluate existence of failure of lower transmission through ho that affect cell mobility. However, this result was not significant as same as that of MAP kinase in the hepatic failure rats. [Histological examination of the remnant liver] The hepatectomized rats were sacrificed and these remnant liver were examined microscopically. Control group was set as no liver damage rats with 84% hepatectomy. Mitotic index after 48hr. of hepatectomy had no significant difference compared with that of control group. Therefore, rats in hepatic failure after hepatectomy might not have had significantly depressed liver regeneration. In addition to above, the previous results were evaluated and analyzed.