A STUDY ON THE MECHANISM OF RADICULAR CYST EXPANSION USING RADICULAR CYST-DERIVED CELLS

1. Prostaglandins （PGE型D21型D2， PGE型D22型D2， TXA型D22型D2） stimulated IL-6，TGF-β1 and PDGF-A gene expression and the synthesis of these proteins by radicular cyst-derived fibroblast-like cells （RC cells）。2. RC-D22-D2的秘密由RC细胞在5-至15-被刺激时与IL-1 α、-1β、PDGF-AA、-BB、EGF或bFGF。Furthermore，IL-1 α-刺激PGE-D22-D2生产被H7 （PKC抑制剂）或dexamethasone.3显著抑制。PGE D22 inhibited DNA synthesis by RC cells， whereas low concentration of TXA D22 stimulated DNA systhesis。PGE-D22-D2 or TXA-D22-D2 somewhat inhibited collagenase production。TXA-D22-D2 significantly stimulated IL-6 production， and PGE-D22-D2 markedly stimulated LIF synthesis。两种前列腺素刺激的IL-8合成是依赖的，和有标记的刺激的MCP-1生产。通过RT-PCR分析的五个激进细胞线条中的细胞因子/生长因子mRNA水平。The cyst lining epithelia明显 ... More 表达白细胞介素-1 α， -1β，白细胞介素-6，白细胞介素-8，白细胞介素-11，TGF-β1， PDGF-A， aFGF和KGF，EGF（c-Met）和白细胞介素-6.5受体。酶基因对各种蛋白质溶液（albumin、Gelatin或transferrin）或hyaluronic acid （HA）溶液的动态压力的影响已被确定。Although蛋白质集中对运动压力中的增加有很小的影响，每个蛋白质的酶消化率对增加运动压力有明显的影响（approx）。8至20 mOSM）。按国家分列的蛋白质解决方案，HA解决方案浓度低（0.1%），已公布批准。10 mOSM在运动压力中增加，因为相同的HA解决方案在酶消化后增加了3 mOSM。活性和晚期聚合酶活性在所有细胞流体标本中都找到，活性聚合酶在65 kDa时与抗人类MMP-8抗体交叉反应。凝胶酶活性也在所有的标本中找到，由运动学检测到的主要双带直径为92 kDa，由运动学检测到的小带直径为72 kDa;那些与反人类MMP-9和-2抗体交叉反应，尊重。所有的酶活性都被MMP抑制剂、O-phenanthroline的补充所阻断。Less（低）

1. Prostaglandins (PGEィイD21ィエD2, PGEィイD22ィエD2, TXAィイD22ィエD2) stimulated IL-6, TGF-β1 and PDGF-A gene expression and the synthesis of these proteins by radicular cyst-derived fibroblast-like cells (RC cells).2. PGEィイD22ィエD2 secretion by RC cells were increased 5- to 15-fold when stimulated with IL-1α, -1β, PDGF-AA, -BB, EGF or bFGF. Furthermore, IL-1α-stimulated PGEィイD22ィエD2 production was significantly inhibited by H7 (PKC inhibitor) or dexamethasone.3. PGEィイD22ィエD2 inhibited DNA synthesis by RC cells, whereas low concentration of TXAィイD22ィエD2 stimulated DNA systhesis. PGEィイD22ィエD2 or TXAィイD22ィエD2 somewhat inhibited collagenase production. TXAィイD22ィエD2 significantly stimulated IL-6 production, and PGEィイD22ィエD2 markedly stimulated LIF synthesis. Both prostanoids stimulated IL-8 synthesis dose-dependently, and marked stimulated MCP-1 production.4. mRNA levels of cytokines/growth factors in five radicular cyst lining epithelia were analyzed by RT-PCR. The cyst lining epithelia apparently … More expressed IL-1α, -1β, IL-6, IL-8, IL-11, TGF-β1, PDGF-A, aFGF, and receptors for KGF, EGF, HGF (c-met) and IL-6.5. Effects of enzyme digestion on osmotic pressure of various protein solution (albumin, gelatin or transferrin) or hyaluronic acid (HA) solution was determined. Although protein concentrations had little effect on the increase in osmotic pressure, enzyme digestion rate of each protein was significantly effective to increase osmotic pressure (approx. 8-20 mOSM). Countrary to protein solution, low concentration of HA solution (0.1%) revealed approx. 10 mOSM increase in osmotic pressure, whreas the same HA solution increased only 3 mOSM after the enzyme digestion.6. Active and latent collagenase activities were found in all the cyst fluid specimens, and active collagenase was cross-reacted with anti-human MMP-8 antibody at 65 kDa. Gelatiase activity was also found in all the specimens, which detected as major doublet bands colse to 92 kDa, and a minor band at 72 kDa by zymography ; those were cross-reacted with anti-human MMP-9 and -2 antibody, respectively. All the enzyme activities were blocked by the addition of an MMP inhibitor, o-phenanthroline. Less