Effect of Magnetic Field on Stage of Cell Division

我们检测了磁场对线粒体活性、细胞呼吸、细胞形态发生、细胞黏附以及细胞内或细胞外离子密度的影响。磁场对线粒体活性的影响作为初步检查，通过将细胞置于低密度胎牛血清的培养液中，检测了使细胞进入低能状态所需的时间，即细胞周期的G0/G1期，以及降低代谢活动水平所需的时间。我们通过用四甲基偶氮唑盐比色法估计细胞代谢来检测磁场对线粒体代谢活性的影响。磁场保持在100[mT]，对照组为0[mT]。四甲基偶氮唑盐比色试验结果表明，线粒体的代谢活性似乎提高了1.5.2倍。磁场对细胞呼吸的影响线粒体的代谢活动被认为是折射…更多地依赖于它的呼吸。在磁场作用下，细胞呼吸增加了1.3倍。换言之，从介质中氧气的变化可以看出，磁场对细胞呼吸的影响很小。磁场对细胞内和细胞外离子密度的影响我们关注细胞内和细胞外液之间的钙离子转运，以考察这一方面。我们检测了当暴露在磁场中时，ATP的变化。当细胞中加入三磷酸腺苷时，细胞的代谢活动就会增强。而暴露在磁场中，即使添加了ATP，其活性也没有增强。磁场对细胞形态发生和细胞黏附的影响利用相敏显微镜和CCD摄像机对细胞形态发生进行成像观察。当暴露在磁场中时，形态发生发展被抑制了约1%。比对照高40%。为了分析抑制作用的因素，我们用结晶紫方法检测了细胞粘附性。结果表明，当暴露在磁场中时，细胞的黏附被抑制了大约10%。比对照高30%。较少

We examined the effectrs of the magnetic field in terms of the mitochondria activity, the cell respiration, the cell morphogenesis, the cell adhesions and intercellular or extracellular of the ion density.1. Effects of the magnetic field on the mitochondria activityAs a preliminary examination, the time required to render the cells to the low energy state, I.e., to the G0/G1 phase of the cell cycle, and to reduce the metabolic activity level, was examined by keeping the cells in the medium with low-density fetal bovine serum. We examined the effect of the magnetic field on the metabolic activity of the mitochondrial by estimating the cell metabolism using the MTT assay. The magnetic field was kept at 100[mT]as well as 0[mT]for the control. The results by MTT assay indicated that the metabolic activity of the mitochondria seems to been enhanced by the factor of 1.5.2. Effects of the magnetic field on the cell respirationThe metabolic activity of the mitochondria is considered to be refl … More ected on its respiration. The cell respiration is increased by the factor of 1.3 by the exposed to the magnetic field. In other words, the change of the cell respiration by the magnetic field is very small, as seen from the change of the oxygen in the medium.3. Effects of the magnetic field on the intracellular and extracellular of ion densityWe concern with the calcium ion transport between the intracellular and the extracellular fluid, in order to examine this aspect. We examined the change of ATP when exposed to the magnetic field. When ATP is added to the cell, the cell metabolic activity is enhanced. While, exposed to the magnetic field, even if, ATP is added, the activity is not enhanced.4. Effects of the magnetic field on the cell morphogenesis and cell adhesionThe cell morphogenesis is observed by imaging by using a phase-sensitive microscope and CCD camera. When exposed to the magnetic field, the morphogenesis development is inhibited by approx. 40% than that of the control. In order to analyze the factor for inhibition, we examined the cell adhesion by the crystal violet assay. It is indicated that, when exposed to the magnetic field, the cell adhesion is inhibited by approx. 30% than that of the control. Less