Establishment of basic property for the development of mobile type drug checker

最近，阻力问题变得更加严重。为了解决这一问题，对该药物进行快速、简便的分析是非常重要的。在法医学领域，用气相色谱、薄层层析、紫外可见光谱、荧光分光光度计法、酶免疫法、放射免疫法、气相色谱-质谱仪等方法分析拖拉物。然而，这些方法在定性和定量分析方面需要一系列的操作步骤。顺便说一句，表面等离子体共振现象是法医学领域最有希望的现象之一，因为利用这一现象，该方法能够快速、准确地检测血液或尿液中的甲基苯丙胺等法律控制物质。首先，为了使其付诸实践，我们一直致力于提高甲基苯丙胺的选择性和敏感度。我们试图准备的系统是流动注射型的，它是用下一个装配隔室1的泵送装置建造的。所有溶液均以每分钟0.05ml的流速通过色谱柱，在室温下分离物质。传感装置采用镀金玻璃显微镜载玻片。传感器在1/15M磷酸二氢钾缓冲液中平衡，pH 7.6，含0.9%NaCl.3监测仪。使用Macintosh计算机对出现的现象进行监测。通过泵入流动池至少10分钟来分析样品。以72°为入射角，在11.1min内获得了清晰的甲基苯丙胺表面等离子体共振共振角曲线。当甲基苯丙胺浓度为25μg/ml时，共振角为0.005。现在，我们正在努力使这一系统更具选择性和便携性。

Recently the drag problem has been becoming more serious. To solve this problem, it is important to analyze this drug easily and rapidly. In forensic science field drag has been analysed by gas and thin layer chromatography ultraviol and fluorescence spectrophotometer, enzyme immunoassay method, radio immunoassay method and gas chromatographic mass spectroscopy. However, these methods require a series of operational procedures in the qualitath and quantitative analysis.By the way, surface plasmon resonance phenomenon is one of the most effective phenomenon full of promise in the field of forensic science, since by availing this phenomenon this method has a ability to detect law controlled substances such as methyl amphetamine in blood or urine, rapidly and accurately. First of all, to make it practice, our efforts has been focussing to detect methyl amphetamine more selective and more sensitive. The system which we tried to prepare was flow injection type and it was constructed with the next assembly compartments.1 Pumping apparatus. All solutions were pumped at the flow rate of 0.05 ml per a minute through the column, by which substances were separated at room temperature.2 Detecting apparatus. As the sensing device, gold-coated glass microscope slide was used. Sensor was equilibrated in 1/ 15 M potassium phosphate buffer, pH 7.6 containing 0.9 % NaCl.3 Monitor apparatus. To monitor arising phenomenon, Macintosh computer was availed. Samples were analysed by pumping through the flow cell for a minimum of 10 minutes. Clear resonance angle curve of Surface plasmon resonance for methyl amphetamine was obtained at 11.1 minutes by applying 72 degree as incident angle. The resonance angle is 0.005 at the concentration of 25 μg/ml methyl amphetamine. Now our efforts are under way to make this system more selective and portable.