The invention of the organism simulated molecular module (ATP generator).

氧化硫硫杆菌（Thiobacillusthiooxidans）是一种具有耐酸能力的硫氧化细菌，它利用细胞外pH（低）和细胞内pH（中性）的pH差获得能量（ATP）。细菌中的耐酸因子似乎是细胞色素c和H^+-ATP酶（ATP合成酶）。细胞色素c：将细菌的无细胞提取液离心分离成膜组分和水溶性组分。细胞色素c为碱性蛋白，存在于细胞膜组分中，分子量为18 kDa，等电点为8.2。水溶性细胞色素c的分子量为10.1 kDa。还原的细胞色素c具有416.5、522和551 nm的吸收光谱。氧化的细胞色素c在409.5 nm处有吸收光谱。等电点为8.5。H^+-ATPase：ATPase存在于细胞膜上，为F_0F_1型。ATP酶的最适反应温度为60℃，最适pH为8.0。随着Mg^<2+>浓度的增加，酶活性增加，在4 mM-Mg ^<2+>时达到最大值。ATP分解活性是氧化硫硫杆菌ATP酶的一个特征性质，它与SO_3 ~（2-<2->）浓度有关。

Thiobacillus thiooxidans, sulfur-oxidizing bacteria with acid resistance, acquires energy (ATP) with the help of the pH gap of extracellar pH (low) and intracellar pH (neutral). The acid-resistant factors in the bacteria seem to be cytochrome c and H^+-ATPase which is ATP synthetase. In this study, these acid-resistant factor were separated, and the property was examined.Cytochrome c : Cell-free extract of bacteria was divided by centrifugation into membrane fraction and water-soluble fraction. Membrane -bound cytochrome c which existed in the membrane fraction was the basic protein, and the molecular weight was 18 kDa, and the isoelectric point was 8.2. The molecular weight of water-soluble cytochrome c was 10.1 kDa. The reduced cytochrome c had the absorption spectrum for 416.5, 522, and 551 nm. The oxidized cytochrome c had the absorption spectrum for 409.5 nm. The isoelectric point was 8.5. H^+-ATPase : ATPase which existed in the cell membrane was the F_0F_1 type. The optimum activity of ATPase was observed in the decomposition of ATP at pH8.0, 60℃. The activity increased with increasing Mg^<2+> concentration, and it became a maximum at 4 mM-Mg^<2+>. As a characteristic property in ATPase of T.thiooxidans, the ATP decomposing activity was dependent on the sulfurous acid (SO_3^<2->) concentration.