Physiologic and molecular biologic studies on normality of bovine nuclear transfer embryos

核移植是一种可能导致大而畸形后代出生的程序。我们用胚胎生理学和分子生物学的方法检查了牛核移植胚胎的正常情况。首先，我们研究了血清补充对受体卵母细胞成熟培养基的影响。有血清或无血清成熟时，可核卵母细胞、融合卵母细胞、卵裂胚胎和囊胚发育的频率相同。其次，我们研究了利用来自妊娠子宫的氨基酸细胞作为供体细胞进行牛核移植的可能性。与羊膜细胞或胎儿成纤维细胞进行核移植，融合卵母细胞、卵裂胚胎和囊胚发育的频率相同。这些结果表明，牛核移植不需要使用血清培养卵母细胞和胚胎，牛羊膜细胞在核移植后可能具有发育能力。用于牛核移植的无血清培养系统应有助于检查核移植胚胎的正常和/或异常。

Nuclear transfer is procedures that can result in the birth of large and abnormal offspring. We examined the normality of the bovine embryos derived from nuclear transfer using the means of embryo physiologic and molecular biologic procedures. First, we examine the effects of the serum supplementation to the maturation medium for the recipient oocytes. The frequencies of enuclearable oocytes, fused oocytes, cleaved embryos and development to the blastocysts were the same for maturation with or without serum. Second, we examine the possibility of using aminotic cells derived from pregnant uterus for donor cells of bovine nuclear transfer. The frequencies of fused oocytes, cleaved embryos and development to the blastocysts were the same for nuclear transfer with amniotic cells or fetal fibroblast cells. These results indicate that there is no need to use serum for oocytes and embryos culture in bovine nuclear transfer, and that bovine amniotic cells may have demonstrate developmental ability after nuclear transfer. Serum- free culture system for bovine nuclear transfer should facilitate examinations to investigate normality and/or abnormality of the embryos derived from nuclear transfer.