Study on the development of PRL and GH cells in the anterior pituitary of neonatal rats

雌二醇处理对新生大鼠垂体前叶PRL和GH细胞发育的影响：首先，测定抗甲胎蛋白抗体对新生大鼠垂体PRL和GH细胞发育的影响。此外，利用免疫细胞化学和RT-PCR技术估计雌二醇（E2）和新生儿血清组分对体外PRL细胞发育的影响。抗甲胎蛋白治疗后血清E2浓度未升高。然而，垂体PRL细胞%的抗afp倾向于增加，而GH细胞%的抗afp倾向于增加。E2对PRL细胞%有剂量依赖性刺激，但对GH细胞%无刺激作用。免疫细胞化学检测新生儿血清部分抑制垂体PRL细胞的发育，PRL基因表达与蛋白表达结果一致。提示E2刺激新生大鼠垂体前叶PRL细胞的发育，这种刺激可能受新生大鼠血清中某种因子的调控。此外，PRL蛋白的翻译可能受到新生儿血清中某个因子的调控。

Effect of estradiol treatment on the development of PRL and GH cells in the anterior pituitary of neonatal rats : First, the effect of anti-alpha fetoprotein antibody on the development of PRL and GH cells in the pituitary of neonate was determined. Furthermore, influences of estradiol (E2) and serum fractions of neonate in PRL cells development in vitro were estimated using immunocytochemistry and RT-PCR.Serum E2 concentration did not elevate by Anti-AFP treatment. However, anti-AFP tended to be increased in PRL cell % of the pituitary but not in GH cell %. E2 was dose-dependently stimulated the increase in PRL cell % but not in GH cell %. While serum fractions of neonate inhibited the development of PRL cell in the pituitary determined by immunocytochemistry, PRL gene expression was in accord with the result of protein expression.These results indicate that E2 stimulates the development of PRL cell in anterior pituitary of neonatal rats, the stimulation may be regulated by a factor(s) in neonatal serum. Moreover, it is possibility that the translation into PRL protein is regulated by a factor(s) in neonatal serum.