THE TARGET GENE FOR PPARα AND PPARγ IN HUVEC

我们研究了过氧化物酶体增殖物激活受体α (PPARα)和PPARγ对培养的人脐静脉内皮细胞（HUVEC）炎症细胞因子的产生和表达以及前列腺素和超氧化物产生相关酶的表达的影响。PPARα和PPARγ显著降低培养基中白细胞介素-1β和-6 mRNA表达及蛋白水平，抑制环氧化酶-2 mRNA表达及蛋白水平。此外，PPARα和PPARγ诱导尼古丁腺嘌呤二核苷酸磷酸（NADPH）氧化酶的22-kD亚基p22phox的mRNA水平和47-kD亚基p47phox的蛋白水平降低。这种独特的抗炎作用，加上它的降血脂作用，可能有利于预防由高脂血症引起的血管并发症。

We examined the effects of peroxisome proliferator-activated receptor α (PPARα) and PPARγ on the production and expression of inflammatory cytokines and on enzyme expression involving prostaglandin and superoxide production in cultured human umbilical vein endothelial cells (HUVEC). PPARα and PPARγ significantly reduced interleukin-1β and -6 mRNA expression and their protein levels in the culture medium, and also inhibited cyclooxygenase-2 mRNA expression and their protein levels. Moreover, the mRNA levels of p22phox, a 22-kD subunit and the protein levels of p47phox, a 47-kD subunit of nicotine adenine dinucleotide phosphate (NADPH) oxidase, was decreased by induction of PPARα and PPARγ. This unique anti-inflammatory effect in addition to its hypolipidemic action, may be beneficial in preventung the vascular complications that are induced by hyperlipidemia.