Perioperative production of vein graft to which transfer anti-arteriosclerosis gene for CABG with gene gun

基因枪已经被用来将基因转移到各种器官中，但还没有成功的基因枪介导的基因转移到心脏的报道。在这项研究中，我们评估了使用基因枪和异体质粒载体进行基因治疗的可能性。基因表达持续6周。上体载体显然有助于长时间的表达。单核细胞或白细胞浸润性很弱。在被轰击的心脏中检测到β-Gal DNA，但在其他器官中没有检测到。基因枪介导的表型载体转入心脏跳动中可能为基因治疗提供一种简单、有效和有用的策略。在糖尿病患者中，左心功能的特征被报道为舒张期功能障碍，但从机械能量学的角度对其心功能的研究尚未见报道。目的：采用大冢长伊万斯-德岛脂肪大鼠(…模型)，研究左心室(LV)机械能学特性。更多的II型糖尿病模型大鼠)全心准备。实验动物为长期组(L-I组，40~46周龄)、长期II组(L-II组，62~66周龄)、长期II组(L-II组，62~66周龄)、对照组(5只)。我们评估了每组的LV机械能量值和SERCA2的Western blotting。VO_2-PVA关系的平均ESPmax、平均斜率和VO_2截距在三组大鼠之间无差异。L-II组最大起搏频率降至240次/分。在240次/分起搏时，L-II组的平均Logistic时间常数显著长于对照组(18.2ms比13.5ms)(P&lt；0.05)。L-II组SERCA2平均蛋白水平显著低于L-I组和对照组。结论：长期高血糖大鼠左心室收缩功能得到很好的保护，外源性钙释放补偿了左心室收缩功能，但SERCA2表达降低，导致左心室收缩功能受损。较少

Gene guns have been used to transfer genes into various organs, but there has been no report of successful gene gun mediated gene transfer into the heart. In this study, we assessed the possibility of gene therapy using a gene gun and an episomal plasmid vector. Gene expression persisted for 6 weeks. The episomal vector apparently contributed to long-lasting expression. Infiltration of monocytes or leukocytes was very faint. The β-gal DNA was detected in bombarded hearts but not other organs. Gene gun mediated transfer of the episomal vector into beating heart may provide a simple, efficient, and useful strategy for gene therapy.In diabetes mellitus (DM) patients, the character of left ventricular (LV) function is reported as diastolic dysfunction, but their cardiac functions in terms of mechanoenergetics have not been studied yet. Purpose : We investigated left ventricular (LV) mechanoenergetics using the excised blood-perfused Otsuka Long-Evans Tokushima Fatty (OLETF) rats (model of … More type II DM model rat) whole heart preparation. Animals were 6 long-term * (L-I ; 40-46 weeks of age) OLETF rats (L-I group), 6 long-term II (L-II ; 62-66 weeks of age) OLETF rats (L-II group) and 5 control LETO rats (control group). We assessed LV mechano-energetics valuses and western blots for SERCA2 in each group. Mean ESPmax, mean slopes and VO_2 intercepts of VO_2-PVA relations did not show any differences among the three rat groups. Maximum pacing rate decreased to 240 beats/min in L-II group. Mean logistic time constants at 240-bpm pacing in L-II group were significantly (P<0.05) longer than the constants in control group (18.2 vs. 13.5 msec). Mean protein level of SERCA2 in L-II group was significantly lower than that in L-I group and control group. Conclusion : We conclude that the lusitropic function is impaired bv depressed expression of SERCA2 in prolonged hyperglycemia rats, although LV systolic function is well preserved and the Ca^<2+> handling is compensated by external Ca^<2+> extrusion. Less