TISSUE ENGINEERING PERICARDIUM

各种血管移植物通常用于心血管组织的重建。然而，目前使用的假体或生物假体材料缺乏生长潜力，因此，随着它们的成熟，随后需要在儿科患者中进行替换。组织工程学伊萨一门新兴学科，它为自体细胞和生物相容性高分子支架提供了替代结构。由于Th构建体含有活细胞，它们可能具有生长、自我修复和自我重塑的潜力，因此通过将4-6 × 10^6的从蒙古犬股静脉获得的混合细胞接种到由聚乙醇酸（PGA）非织造织物片和l-丙交酯和己内酯的共聚物（N=4）组成的管状可生物降解聚合物支架上来制备组织工程血管自体移植物（TEVA）。7天后，用TEVA替换相同犬的下腔静脉（IVC）。三、四之后。术后5、6个月行血管造影， ...更多信息 虚构的对植入的TEVA进行大体和免疫组织学检查。植入的TEVA未显示狭窄或扩张迹象。TEVA内未发现血栓。即使没有任何抗凝治疗。在所有标本中未观察到聚合物支架的残留，总体外观与天然IVC相似。免疫组织化学染色显示在TEVA的管腔表面存在因子VIII阳性裸细胞。此外，在α-平滑肌肌动蛋白和结蛋白阳性细胞存在的地方观察到病变。植入的TEVA含有足够的细胞外基质，没有显示闭塞或动脉瘤形成。另外。发现内皮细胞排列在每个TEVA的腔表面。这些结果有力地表明，“理想的”静脉移植物具有抗血栓形成可以产生。使用TEtedmique，成功地重建了周围肺动脉在一个4岁的女孩使用自体静脉细胞。用TEvessel移植物重建闭塞的肺动脉，无术后并发症发生。随访血管造影，移植血管完全通畅。以相同的方式，在动物模型中检查使用生物可降解聚合物片材的自体心包。心包粘连相对较轻的假体材料。由于我们的实验室和临床经验是相当令人鼓舞的，我们建议TE方法可能会发挥重要的作用，作为一种替代方法，移植和使用人造器官的儿科心血管手术的fldd。少

Various vascular grafts am commonly used in the reconstruction of cardiovascular tissues. However, cunently used prosthelic or bioprosthetic materials lack giowth potential and, therefore, subsequently requie replacement in pediatric Talients as they matrure. Tissue engineering (YE) isa new discipline that offers the potential to create replacement structures ficm autologous cells and bkxlegiadable polymer scaffolds. Because Th constructs contain living cells, they may have the potential to grow, self-repair, and self-remodel, Tissue Engineered Vascular Autografts (TEVAs) were made by seeding 4-6 x 10^6 of mixed cells obtained fiom femoral veins of mongiel dogs onto tube-shaped biodegradable polymer scaffolds composed of a polyglyecolic acid (PGA) non-woven fabric sheet and a co-polymer of l-lactide andcapolactone (N=4). After 7 days, the inferior vena cavas (IVCs) of the same dogs were replaced with TEVAs. After 3,4. 5 and 6 months, angiographies were perfomed, and the dogs were sacii … More ficed. The implanted TEVAs were examined both grossly and immunohistologically. The implanted TEVAs showed no evidence of stenosis or dilatation. No thrombus was found inside the TEVAs. even without any anticoagulation therapy. Remnants of the polymer scaffolds weie not observed in all specimens, and the overall gross appearance appeared similar to that of native IVCs. Inimunohistological staining revealed the presence of Factor VIII positive nudcated cells at the luminal surface of the TEVAs. In addition, lesions were observed where α-smooth muscle actin and desmin positive cells existed. Implanted TEVAs contained a sufficient aniotnit of extracellular matrix, and showed neither occlusion nor anetuysmal formation. In addition. endothelial cells were found to line the luminal surface of each TEVA. These results strongly suggest that 'ideal' venous grafts with anti-thrombogenicity can be produced. Using theTEtedmique, a peripheral pulmonary artery was successfully reconstructed in a 4 year-old girl using autologous venous cells. The occluded pulmonary artery was reconstructed with the TEvessel graft No postojemtive complications occurred On follow-up angiography, the transplanted vessels were noted to be completely patent. In the same manner, autologous pericardium using biodegadable polymer sheet was examined in an animal model. Pericardial adhesion was relatively mild companing to the prosthetic materials. Because both our laboratory and clinical experiences are quite encouraging, we suggest that the TE approach may play an important ole as an alternative metohd to transplantation and to the use of artificial organs in the fldd of pediatric cardiovasucular surgery. Less