ROLE OF STEM CELL FACTOR IN OCULAR SURFACE

1) SCF对大鼠角膜上皮创面愈合的作用外用SCF可显著促进Wistar大鼠角膜上皮创面愈合。更有趣的是，与+/+大鼠相比，缺乏c-kit受体的Ws/Ws大鼠的角膜上皮愈合明显延迟，这表明内源性SCF可能在角膜上皮愈合中发挥作用。2) SCF对小鼠角膜上皮创面愈合的作用局部给予SCF的C57/BL小鼠角膜上皮创面愈合也显著促进。此外，与对照组相比，scf缺乏和c-kit受体缺乏的动物的上皮愈合明显延迟。在SCF缺陷小鼠中，局部应用SCF几乎完全恢复了这种延迟，而在c-kit受体缺陷小鼠中则没有发生这种现象。3) SCF对角膜上皮细胞粘附的影响SCF可显著促进角膜上皮细胞粘附，且呈剂量依赖性。此外，添加抗SCF抗体可有效阻断细胞粘附，进一步支持SCF参与角膜上皮细胞粘附。4)人结膜乳头SCF/c-kit受体的免疫组化发现，aescf主要定位于睑结膜成纤维细胞。在结膜乳头血管内皮细胞上也发现了SCF的免疫反应性。c-kit受体在结膜上皮和肥大细胞中表现出免疫反应性。5)变应性疾病患者泪液中SCF浓度正常志愿者和季节性变应性结膜炎患者泪液中均未检出SCF，而春性角膜结膜炎患者泪液中SCF浓度极高（483±286 pg/ml），提示SCF可能通过肥大细胞的活化和增殖在致病机制中起作用。少

1) Role of SCF on the corneal epithelial wound healing in ratsUpon topical SCF administration, corneal epithelial wound healing was significantly promoted in Wistar rats. More interestingly, corneal epithelial healing of Ws/Ws rats which were deficient with c-kit receptors was significantly delayed in comparison with +/+ rats, suggesting that endogenous SCF might play a role in corneal epithelial healing.2) Role of SCF on the corneal epithelial wound healing in miceCorneal epithelial wound healing was also significantly promoted in C57/BL mice receiving topical SCF administration.In addition, the epithelial healing was significantly delayed in SCF-deficient as well as c-kit receptor-deficient animals as compared with controls. This delay was almost completely recovered in SCF-deficient mice by topical application of SCF, while such phenomenon did not occur in c-kit receptor-deficient mice.3) Effect of SCF on corneal epithelial cell adhesionSCF as sown to significantly promote the corne … More al epithelial cell adhesion in a dose-dependent manner. Moreover, the cell adhesion was efficiently blocked by adding anti-SCF antibodies, further supporting the involvement of SCF in corneal epithelial cell adhesion.4) Immunohistochemistry of SCF/c-kit receptor in human conjunctival papillaeSCF was found mainly localized in the fibroblasts of the palpebral conjunctiva. The immunoreactivity of SCF was also recognized at the vascular endothelial cells of the conjunctival papillae. The immunoreactivity of c-kit receptor was demonstrated in the conjunctival epithelium and mast cells.5) SCF concentration in tear fluids of the patients with allergic disordersSCF was not detectable in the tear fluids of normal volunteers nor seasonal allergic conjunctivitis patients, while its concentration was extremely high with the patients with vernal keratoconjunctivitis (483±286 pg/ml), suggesting that SCF might be operative in the pathogenic mechanism by way of activation and proliferation of mast cells. Less