Study of graft-infiltrating cells in the rat small bowel allograft using FK-506 or FTY-720

背景：为了阐明新型高效免疫抑制剂FTY-720在小肠移植(SBTx)中的细胞机制，我们研究了移植物浸润性细胞中的淋巴细胞亚群。材料和方法：在DA(RT1a)与Low(RT11)完全错配的大鼠品系组合中进行异位SBTx。受者分为两组：GO组(无免疫抑制剂)(n=5)，GO+FTY-720治疗(1 mg/kg/d，0~5d口服)(n=5)。第6天收集移植物浸润性细胞，用WE/25(辅助性T细胞)、OX-8(CD8；细胞毒性T细胞)、OX-39(CD25；IL-2R)和OX-33(CD45RA；B细胞)进行流式细胞术分析淋巴细胞亚群。CD8阳性细胞(G0：21.2±4.3us G0：14.2±3.1%，p&lt；0.05)，CD45RA阳性细胞(G0：38.1±7.9us G0：29.0±4.9%，p&lt；0.05)，CD8阳性细胞(Go：28.1±7.6us G0：12.8±3.7%，p&lt；0.01)，CD25阳性细胞(G0：5.1±1.6us G0：2.3±1.7%，p&lt；斑块中CD45RA阳性细胞(Go：20.6±6.9us Go：71.4±5.4%，p&lt；0.01)，上皮内淋巴细胞中CD4阳性细胞(Go：18.6±4.4us G0：25.5±0.9%，p&lt；0.01)，CDS阳性细胞(G0：59.1±9.2us G0：34.0±12.2%，p&lt；0.01)，CD4阳性细胞(G0：44.9±3.5us 65.7±8.4%，p&lt；0.01)，CDS阳性细胞(G0：44.9±3.5us 65.7±8.4%，p&lt；0.01)。CD25阳性细胞(G0：5.1±3.0us G0：1.0±0.7%，p&lt；0.05)，CD45RA阳性细胞(G0：9.7±1，8us G0：2.5±0.3，3%，p&lt；0.01)。结论：FTY-720对移植物固有层淋巴细胞的免疫抑制作用可能与抑制细胞毒性T细胞和IL-2R阳性细胞有关。

Background: To elucidate the cellular mechanism of FTY-720, a new potent immunosuppressant, in small bowel transplantation (SBTx), we studied lymphocyte sub populations in graft infiltrating cells.Materials and methods : Heterotopic SBTx was performed in a fully mismatched DA (RT1a) to LOW (RT11) rat strain combination. Recipients were divided into two groups : GO) no immunosuppressant(n=5), GO) FTY-720 treatment (1mg/kg/day, oral garage from day 0 to day 5)(n=5). The graft infiltrating cells were harvested on day 6 and lymphocyte sub populations were analyzed by flow cytornetry with the following monoclonal antibodies ; WE/25(CD4 ; helper T cell), OX-8(CD8 ; cytotoxic T cell), OX- 39(CD25 ; IL-2R), and OX-33(CD45RA ; B cells).Results : GO demonstrated typical histology of rejection, while GO did not show any signs of rejection. The following data showed significant difference : CD8 positive cells (G0:21.2±4.3 us G0:14.2±3.1 %, p<0.05), CD45RA positive cells (G0:38.1±7.9 us G0:29.0±4.9 %, p<0.05) in the mesenteric lymph nodes ; CD8 positive cells (GO:28.1±7.6 us G0:12.8±3.7 %, p<0.01), CD25 positive cells (G0:5.1±1.6 us G0:2.3±1.7 %, p<0.05), CD45RA positive cells (GO:20.6±6.9 us GO:71.4±5.4 %, <0.01) in the pyres patches ; CD4 positive cells (GO:18.6±4.4 us G0:25.5±0.9 %, p<0.01), CDS positive cells (G0:59.1±9.2 us G0:34.0±12.2 %, p<0.01) in the intraepithelial lymphocytes ; CD4 positive cells (G0:44.9±3.5 us 65.7±8.4 %, p<0.01), CDS positive cells (G0:32.9±3.4 us G0:14. 4±1.7 %, p<0.01), CD25 positivecells (G0:5.1±3.0 us G0:1.0±0.7 %, p<0.05), CD45RA positive cells (G0:9.7±1,8 us G0:2.5±0,3 %, p<0.01) in the lamina propria lymphocytes.Conclusion: The immunosuppressive effect of FTY-720 in SBTx may be due to the depletion of cytotoxic T cells and IL-2R.positive cells in the graft infiltrating cells.