Histological study of experimental murine amyloidosis

淀粉样变性的特征是淀粉样纤维沉积在各种器官和口腔组织的细胞外基质中，无论是在动物还是在人类。在实验性小鼠淀粉样变性中，通过组织学研究来检查淀粉样变性的产生。雌性小鼠被给予0.5ml的淀粉样增强因子(AEF)和0.2ml的丁型支原体弗氏完全佐剂(FCA)的乳剂皮下注射。在乙醚麻醉下处死小鼠，从心脏取血。用小鼠血清淀粉样蛋白免疫试剂盒检测血清淀粉样蛋白A(SAA)，用AEF和淀粉样蛋白培养巨噬细胞。多聚甲醛固定石蜡切片，刚果红、H-E、PAS、柏林蓝染色，抗淀粉样蛋白P组分、抗巨噬细胞F4/80免疫组化染色。随后，用电子显微镜观察固定在Karnovsky液中的组织的超微结构，在脾的滤泡周围带和边缘带诱导淀粉样变性。脾、肝组织中SAA含量、淀粉样蛋白P组分及巨噬细胞数均增加。位于脾边缘带和红髓的巨噬细胞增多，含有蛋白多糖、铁离子(Fe~(2+)和Fe~(3+))和淀粉样蛋白P成分。淀粉样蛋白的沉积是由淀粉样蛋白P组分、硫酸软骨素蛋白多糖、硫酸乙酰肝素蛋白多糖和淀粉样蛋白的蛋白组成的淀粉样纤维组成。淀粉样变性的形成与巨噬细胞和成纤维细胞有关。

Amyloidosis is characterized by the deposition of amyloid fibrils in the extracellular matrix of various organs and oral tissues in animals as well as humans. In the experimental murine amyloidosis, the production of amyloidosis was examined by carrying out histological study.Female mice were given an intraperitoneal injection of 0.5 ml of amyloid enhancing factor (AEF) and a subcutaneously injection of 0.2 ml of an emulsion of M.Butyricum in Freund's complete adjuvant (FCA). The mice were sacrificed under ether anesthesia and blood was taken from the heart. Serum amyloid A (SAA) was measured using a Cytoscreen/Immunoassay kit for mouse SAA.The peritoneal macrophages were cultured with AEF and protein of the amyloid. Paraffin sections of the tissues fixed in paraformaldehyde were stained with Congo red, H-E, PAS and Berlin Blue and were immunostained with anti-amyloid P component, anti-macrophages F4/80. Subsequently, the ultrastructures of tissues fixed in Karnovsky's solution were examined with an electron microscope.The amyloidosis was induced in the perifollicular zone and marginal zone in spleen. SAA levels, amyloid P component and macrophage numbers of spleen and liver increased. The increased macrophages located in marginal zone and red pulp in spleen contained proteoglycan, ferric ion (Fe^<3+>) and amyloid P component. The deposition of amyloid is composed of the amyloid fibrils which are made up of amyloid P component, chondroitin sulfate proteoglycan, heparan sulfate proteoglycan and protein of the amyloid. The formation of amyloidosis is concerned with the macrophages and fibroblasts.