Clinical significance of lineage crossover in leukernic cells

1.采用半套式聚合酶链式反应和聚丙烯酰胺凝胶电泳法对40例初治急性髓系白血病患者的白血病细胞免疫球蛋白重链基因重排的互补决定区3(CDR3)进行了分析。34例患者(85%)检测到克隆性IgH基因重排。对克隆性重排患者的IgH基因CDR3DNA序列进行分析。在分析的46个序列中，有30个序列(65%)具有共同序列。该序列为5‘ATTACTAGATA3’。已在互联网上的V-Base数据库中注册为“D3-22”。目前还没有澄清这种现象是如何产生的，以及它具有什么样的生物学意义。针对重组激活基因开发的进一步研究可能会澄清这一现象。我们在B淋巴系恶性肿瘤患者血清DNA中发现克隆性IgH基因重排。可望将该方法应用于淋巴样恶性肿瘤的临床实验室检查。

1. We have analyzed complementarity-determining region 3(CDR3) of immunoglobulin heavy (IgH) chain gene rearrangement in leukerhic cells fron 40 untreated acute myeloid leukemia patients using semi-nested PCR method and polyacrylamide gel electrophoresis.2. Clonal IgH gene rearrangement was detected in 34 patients (85%).3. IgH gene CDR3 DNA sequence was analyzed in patients showing clonal rearrangement. Thirty sequences (65%) out of 46 analyzed had common sequence.4. This sequence was 5 'ATTACTAGATA3 ' and has. Been registered as "D3-22" in V-Base Database in the Internet.5. It was not clarified how this phenomenon was caused and what kind of biological significance it had. Further research focusing recombination activating gene development may clarify this phenomenon.6. We have found clonal IgH gene rearrangement in DNA from serum of patients with B lymphoid malignancy. It is anticipated that the application of this method in clinical laboratory examination in the case of Blymphoid malignancy.