Establishment of easy detection method of peripheral blood hematologic stem cells by using hematology analyzer and its application for determination of collection volume and harvest timing.

我们研究了应用于SE-9000或XE-2100（Sysmex）的未成熟细胞信息（IMI）通道检测外周血或收获样本中干细胞的有用性。(1)外周血造血干细胞经G-CSF化疗动员至外周血，用造血祖细胞（HPC）监测程序检测IMI细胞总数或HPC，可作为判断造血干细胞采集时机的良好指标。虽然IMI细胞偶尔会产生假阳性结果，但HPC与CD 34阳性细胞相关性良好。当白细胞计数小于20000/μL时，认为40/μL HPC是获得1× 10 e6/kg CD 34阳性细胞的指标。当白细胞计数大于此值时，HPC细胞应大于60-80个/μL。(2)收获汁用CS3000和Spectra两种方法获得的收获汁中HPC数量与CD 34细胞数量之间存在显著相关性。由于HPC/CD 34比值在不同的病例中不同。我们研究了HPC/CD_（34）比值与CD_（34）阳性细胞数、血小板数、RANTES、P-选择素和白细胞介素8浓度的关系，结果表明它们之间无明显关系。HPC/CD 34比值在每种情况下在2或3个连续收获日期间相对恒定，表明HPC计数对临床使用的有用性。RANTES和P-选择素的浓度与血小板数量显著相关，并被认为是从活化的血小板中释放出来的。

We studied about the usefulness of Immature Cell Information (IMI) channel applied to SE-9000 or XE-2100 (Sysmex) on the detection of stem cells both in peripheral blood or harvested samples.(1) Peripheral bloodHematopietic stem cells were mobilized to peripheral blood by chemotherapy with G-CSF.Total IMI cells or hematopoietic progenitor cells (HPC) detected by HPC monitor program were proved to be good indicators to decide the harvest timing. Although IMI cells occasionally made false positive results, HPC correlated well with the CD34 positive cells. When the leukocyte count was less than 20000/μL, 40/μL of HPC was considered to be an indicator to obtain 1×10e6/kg CD34 positive cells. When the leukocyte count was more than that, HPC cells should be more than 60-80/μL.(2) Harvest juiceThere was a significant relationship between HPC number and CD34 cell number in both harvest juice obtained by CS30O0 or Spectra. As HPC/CD34 ratio was different in case by case fashion. we studied about the relationship between HPC/CD34 ratio and the number of CD34 positive cells, or platelets, or the concentrations of RANTES, p-selectin or interleukin 8, resulting in no apparent relation between them. HPC/CD34 ratio was relatively constant in each case during 2 or 3 consecutive harvesting days, suggesting the usefulness of HPC count for clinical usage. The concentrations of RANTES or p-selectin were significantly correlated with platelet number and considered to be released from activated platelets.