Fundamental and clinical studies of flow Cytometric analysis of activated plateles using Activation in Patients with Sleep Apnea Syndrome

睡眠呼吸暂停综合征（SAS）与血栓性疾病（如脑血栓形成和心肌梗死）的风险增加有关。本研究旨在探讨血小板活化在SAS血栓性并发症发生发展中的作用。使用柠檬酸盐全血中的活化依赖性单克隆抗体（MoAb），通过三色流式细胞术评估血小板活化。用PerCP-MoAb-CD 61荧光试剂检测所有血小板，用FITC-PAC 1和PE-MoAb-CD 62 P荧光试剂检测活化血小板，SAS患者血小板活化依赖性MoAb阳性率明显高于正常对照组（PAC 1 63.5± 24.8%，CD 6 2 P 4.8± 5.3%，平均值±SD）与健康对照受试者（PAC 1 18.4± 9.4%，CD 6 2 P 0.8± 0.5%，p<0.0001）相比。总之，这两种活化依赖性标志物在SAS患者的血小板表面上表达增加。血小板活化可能在SAS血栓性并发症的发病机制中起重要作用。

Sleep apnea syndrome (SAS) is associated with increased risk of thrombotic disorders such as cerebral thrombosis and myocardial infarction. In this study we sought to elucidate the role of platelet activation in the evolution of thrombotic complications in SAS.Sixty two patients with SAS and nineteen normal volunteers were enrolled in this study. Platelet activation was assessed by three color flow cytometry using activation-dependent monoclonal antibodies (MoAb) in citrated whole blood. One fluorescent reagent, PerCP-MoAb-CD61 was used to identify all the platelets while two other fluorescent reagents, FITC-PAC 1 and PE-MoAb-CD62P were used to detect activated platelets.The percentage of platelets positive for activation dependent MoAb was significantly higher in patients with SAS (PAC 1 63.5±24.8%, CD 6 2P 4.8±5.3%, mean±SD), as compared with healthy control subjects (PAC 1 18.4±9.4%, CD 6 2P 0.8±0.5%, p<0.0001). In conclusion, both activation dependent markers are increasingly expressed on the platelet surface in SAS patients. It can be suspected platelet activation might play a crucial role in the pathogenesis of thrombotic complications in SAS.