Effect of free cathecholamine and diet on plasma sulpho-conjugated catecholamine concentration and energy metabolism

本研究的目的是研究1)正常受试者中硫代共轭儿茶酚胺（CA-S）的昼夜变化，2)饮食、吸烟和运动对CA-S浓度的影响，3)碳水化合物饮食对运动后过量耗氧量（EPOC）的影响。男性12例（年龄27±7岁）；6名吸烟者和6名非吸烟者分别参加了第一和第二项研究。受试者在实验室中进行了24小时的日常生活研究。在第三项研究（运动试验）中，另外6名男性受试者（25±4岁）以68%的最大摄氧量在循环计力器上运动60分钟。吸烟和轻度运动（乒乓球）后血浆硫酸去甲肾上腺素（NA-S）、硫酸肾上腺素（AD-S）和硫酸多巴胺（DA-S）浓度无明显变化。进食后，血浆DA-S浓度升高，但NA-S和AD-S浓度与进食无关。三种CA-S浓度均在深夜（凌晨1点）达到最高值。高碳水化合物饮食组与正常混合饮食组在68%最大摄氧量下运动60 min后的EPOC值无显著差异。

The purpose of this study was to examine 1) circadian variations of sulfoconjugated catecholamines (CA-S) in normal subjects, 2) the effect of diet, smoking and exercise on CA-S concentrations, 3) the effect of the carbohydrate diet on excess post exercise oxygen consumption (EPOC).Twelve male subjects (age ; 27±7 yrs) ; 6 smoker and 6 non-smoker subjects, participated in the first and second study. For 24 hour, the subjects studied during a daily life in the rabolatory. Other 6 male subjects (25±4 yrs) exercised on a cycle ergometer at 68% of their maximal oxygen uptake for 60-min in third study (exercise trial). There was no change in concentration of plasma noradrenaline sulfate (NA-S), adrenaline sulfate (AD-S) and dopamine sulfate (DA-S) after smoking and light exercise (table tennis). Following the consumption of the meal, plasma DA-S concentration increased, but concentrations of NA-S and AD-S could not be related to eating. All three CA-S concentrations showed the highest values at late evening (at 1 a.m.). There was no significant difference in magnitude of EPOC after 60-min exercise at 68% of maximal oxygen uptake between the high carbohydrate diet trial and normal mixed diet trial.